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| Status |
Public on Jul 30, 2014 |
| Title |
Gene expression profiles of two lung squamous cell carcinoma cell lines treated with SOX2 siRNA |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
SOX2 is a transcription factor essential for self-renewal and pluripotency of embryonic stem cells. Recently SOX2 was found overexpressed in the majority of the lung squamous cell carcinoma (SQC), in which it acts as a lineage-survival oncogene. However, downstream targets/pathways of SOX2 in lung SQC cells remain to be identified. In order to identify genes/pathways likely to be downstream of SOX2, we conducted SOX2 silencing experiments in LK2 and NCI-H520 (H520 thereafter), two SOX2-abundant lung SQC cell lines and analyzed global gene transcription changes by gene expression microarray assay.
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| Overall design |
Each of H520 and LK2 cell lines was treated with either pooled siRNAs of SOX2 or non-silencing (control) siRNAs. After 48 h, cells were harvested and totoal RNA extracted for gene expression microarray analysis using Illumina HumanHT12 v3 BeadChip.
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| Contributor(s) |
Jiang SS, Chang IS |
| Citation(s) |
24477565 |
| Submission date |
Jul 15, 2013 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Shih Sheng Jiang |
| E-mail(s) |
ssjiang@nhri.org.tw
|
| Organization name |
National Health Research Institutes
|
| Department |
National Institute of Cancer Research
|
| Street address |
No. 35 Keyan Road, Zhunan County
|
| City |
Miaoli County |
| ZIP/Postal code |
350 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA212043 |
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