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Series GSE48988 Query DataSets for GSE48988
Status Public on Jul 01, 2014
Title Epigenome analysis of normal colon mucosa samples
Organism Homo sapiens
Experiment type Methylation profiling by array
Summary To investigate whether lifestyle factors modulate the stability of gene promoter methylation in the normal aging colonic mucosa, we performed genome-scale DNA methylation profiling of 178 normal colon biopsies using the Illumina Infinium DNA methylation assay, which assesses the DNA methylation status of 27,578 CpG sites located at the promoter regions of 14,495 protein-coding genes. We identified Aspirin use and hormonal replacement therapy (HRT) suppress, whereas a high body mass index (BMI) and smoking promote age-related hyermethylation. Many of these loci modulated by lifestyle in the healthy colon mucosa coincide with loci hypermethylated in colorectal cancers and down-regulated in adenomas. The data show that lifestyle modulates the stability of DNA methylation in the aging colonic epithelium and thereby impacts the rate of evolution of cancer methylomes.
 
Overall design Bisulphite converted DNA from the 178 samples were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2
 
Contributor(s) Noreen F, Röösli M, Gaj P, Pietrzak J, Weis S, Urfer P, Regula J, Schär P, Truninger K
Citation(s) 24973978
Submission date Jul 17, 2013
Last update date Jan 02, 2015
Contact name Faiza Noreen
E-mail(s) faiza.noreen@unibas.ch
Organization name University of Basel
Department Department of Biomedicine
Street address Mattenstrasse 28
City Basel
ZIP/Postal code 4058
Country Switzerland
 
Platforms (1)
GPL8490 Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2)
Samples (178)
GSM1191108 genomic DNA from normal colon biopsies 56C
GSM1191109 genomic DNA from normal colon biopsies 137A
GSM1191110 genomic DNA from normal colon biopsies 373C
Relations
BioProject PRJNA212703

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE48988_RAW.tar 5.8 Mb (http)(custom) TAR
GSE48988_non-normalized.txt.gz 25.6 Mb (ftp)(http) TXT
Processed data included within Sample table
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