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| Status |
Public on Sep 01, 2013 |
| Title |
Global analysis of NFATc1 binding and histone marks in VEGF-activated endothelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We performed the newly mapping of genome-wide NFATc1 binding events in VEGF-stimulated primary cultured endothelial cells, by chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq). Combined NFATc1 ChIP-seq profile and the epigenetic histone marks revealed that predominant NFATc1-occupied peaks were overlapped with promoter marking but not silencer marking. DNA microarrays with NFATc1 expression or knockdown indicated the predominant NFATc1 binding targets were correlated with induced patterns.
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| |
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| Overall design |
Examination of NFATc1 and two different histone marks in HUVEC in the presence/absense of VEGF.
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| |
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| Contributor(s) |
Suehiro J, Kanki Y, Miura M, Manabe Y, Aburatani H, Kodama T, Minami T |
| Citation(s) |
25157100 |
| Submission date |
Jul 31, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Jun-ichi Suehiro |
| Organization name |
Kyorin University School of Medicine
|
| Department |
Department of Pharmacology and Toxicology
|
| Street address |
6-20-2, Shinkawa
|
| City |
Mitaka |
| State/province |
Tokyo |
| ZIP/Postal code |
181-8611 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE49429 |
Genome-wide approaches reveal functional VEGF-inducible NFATc1 binding to the angiogenesis-related genes in endothelium |
|
| Relations |
| BioProject |
PRJNA215174 |
| SRA |
SRP028803 |
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