|
| Status |
Public on Jun 19, 2014 |
| Title |
Replication timing of siRNA control and siRNA PolQRKO cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
The human A-family DNA polymerase θ (Pol q) is a large, multidomain enzyme whose physiological function is still unclear despite its in vitro translesion synthesis capacity in front of DNA damage and its involvement in some features of DNA repair after external stress. Here we present evidence that Pol q holds a novel role in the absence of external stress as a critical determinant of the replication timing program in human cells. Pol q binds to chromatin at early G1 and is required for proper formation of pre-replicative complexe and replication origin activation. Pol q-depleted cells show modified spatial organization of chromatin-loop structures at replication factories. Genome-wide analysis of replication timing shows delayed replication of a part of early replicating domains and advanced replication of a part of late replicating domains following Pol q depletion. Our results identify Pol q as one of the first critical human factors discovered in the replication timing programme.
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| Overall design |
Two-condition experiment, siRNA control vs. siRNA polQ cells. Biological replicates: 2 control replicates, 2 transfected replicates.
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| Contributor(s) |
Fernandez-Vidal A, Guitton-Sert L, Cadoret J, Baldacci G, Cazaux C, Hoffmann J |
| Citation(s) |
24989122 |
| Submission date |
Aug 09, 2013 |
| Last update date |
Sep 19, 2014 |
| Contact name |
Jean-Charles Cadoret |
| E-mail(s) |
jean-charles.cadoret@ijm.fr
|
| Organization name |
CNRS/ Université de Paris
|
| Street address |
15 rue hélène Brion
|
| City |
Paris |
| ZIP/Postal code |
75013 |
| Country |
France |
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| Platforms (1) |
| GPL10123 |
Agilent-022060 SurePrint G3 Human CGH Microarray 4x180K (Feature Number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA214750 |
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