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| Status |
Public on Oct 24, 2013 |
| Title |
Regulation of Gamma globin gene expression by AT2 and its associated proteins through the cAMP response element. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The upstream Gg-globin cAMP-response element (G-CRE) plays a role in regulating Gg-globin expression through binding of CREB1, cJun and ATF2. In this study we identified the ATF2 DNA-binding partners. ATF2 knockdown resulted in a significant reduction of g-globin expression accompanied by decreased ATF2 binding to the G-CRE. By contrast, stably expressed ATF2 in K562 cells increased g-globin, which was reduced by ATF2 knockdown. Moreover, a similar role for ATF2 on g-globin expression was observed in primary erythroid progenitors. To understand the role of ATF2 in g-globin expression, chromatographically purified G-CRE/ATF2-interacting proteins were subjected to mass spectrometry analysis; binding partners included CREB1, cJun, Brg1, and histone deacetylases among others. Co-immunoprecipitation and chromatin immunoprecipitation assay demonstrated interaction of these proteins with ATF2 in CD34+ cells undergoing erythroid differentiation which was correlated with g-globin expression during development. These results suggest synergism between developmental stage-specific recruitments of the ATF2 protein complex and expression of g-globin during erythropoiesis. Microarray studies in K562 cells support additional roles for ATF2 in hematopoiesis and chromatin remodeling
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| Overall design |
Total RNA was isolated from K562 cells in culture following transfection with scrambled siRNA or ATF2 siRNA following which RNA was isolated and gene expression monitored
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| Contributor(s) |
Pace B, Karmakar S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 23, 2013 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Subhradip Karmakar |
| Organization name |
U of Chicago
|
| Department |
Genetics
|
| Street address |
900 E 57th Street
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
|
| Samples (4)
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| Relations |
| BioProject |
PRJNA216932 |
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