|
| Status |
Public on Jul 30, 2014 |
| Title |
Gene expression profiles of NIH3T3 cells stably transfected with the plasmids encoding the wild type PreS/S gene or the nonsense mutant gene (sW182*) of HBV. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Background and Aims: Whether hepatitis B virus (HBV) could play a direct role in hepatocarcinogenesis remains uncertain. The 3' end nonsense mutations of HBV PreS/S gene have been found to encode transcriptional transactivation activity, suggesting these mutations may contribute to HBV-associated oncogenesis. Recently, we have identified a potent oncogenic HBV surface (S) gene nonsense mutant sW182*. Results: Gene expression microarray study revealed that sW182* mutant was significantly enriched by gene sets associated with cell cycle regulation, DNA repair, or genome instability. The transforming growth factor-induced (TGFBI) gene was downregulated in the sW182* mutant clones, and irresponsive to TGF- treatment. The level of Cyclin D1, a negatively regulated TGFBI target, was highly elevated in sW182* mutant cells. Exogenous expression of TGFBI alleviated the oncogenic activity of sW182* in mouse xenograft study. In human HBV-related HCC cancerous tissue, expression of TGFBI was downregulated in 25 of the 55 (45%) patients. Conclusions: Dysregulation of transforming growth factor-induced (TGFBI) gene is involved in the oncogenic activity of the sW182* mutant of hepatitis B virus S gene. This has never been described before.
|
| |
|
| Overall design |
NIH3T3 cells were stably transfected with plasmids encoding the wild type PreS/S gene or the nonsense mutant (sW182*) of HBV PreS/S gene. Totoal RNAs were extracted from those stable clones for gene expression microarray analysis using Illumina MouseRef-8 V2 BeadChip.
|
| |
|
| Contributor(s) |
Jiang SS, Huang SF |
| Citation(s) |
24662304 |
| Submission date |
Aug 26, 2013 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Shih Sheng Jiang |
| E-mail(s) |
ssjiang@nhri.org.tw
|
| Organization name |
National Health Research Institutes
|
| Department |
National Institute of Cancer Research
|
| Street address |
No. 35 Keyan Road, Zhunan County
|
| City |
Miaoli County |
| ZIP/Postal code |
350 |
| Country |
Taiwan |
| |
|
| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA216982 |
Less...
More...