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Series GSE5040 Query DataSets for GSE5040
Status Public on Jun 14, 2006
Title Polyamides alleviate transcription inhibition associated with long GAA•TTC repeats in Friedreich’s ataxia
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Lymphoblast cells from a patient with Freidriech's Ataxia were incubated with pyrrole-imidazole polyamides targeted to the GAA triplet repeat in the intron 1. The polyamides were shown in cell culture to increase levels of endogenous frataxin mRNA. A normal sibling derived lymphoblast cell line was used as a control.
Keywords: human lymphoblast cells
 
Overall design Normal (GM15851) and patient (GM15850) cell lines were incubated in the presence of match polyamide FA1 at 1uM, 2uM or mismatch polyamide FA2 at 2uM for 7days prior to RNA purification and microarray analysis.
 
Contributor(s) Burnett R, Melander C, Puckett J, Dervan P, Gottesfeld J
Citation(s) 16857735
Submission date Jun 09, 2006
Last update date Mar 25, 2019
Contact name Ryan Stephen Burnett
E-mail(s) rburnett@scripps.edu
Phone 858-784-8920
Fax 858-784-8965
Organization name The Scripps Research Institute
Department Molecular Biology
Lab Gottesfeld
Street address 10550 N. Torrey Pines Road, MB-27
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (24)
GSM113678 GM15851 control cells untreated 1
GSM113802 GM15851 control cells untreated 2
GSM113803 GM15851 control cells untreated 3
Relations
BioProject PRJNA96673

Download family Format
SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE5040_RAW.tar 185.2 Mb (http)(custom) TAR (of CEL)

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