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| Status |
Public on Dec 31, 2006 |
| Title |
Effect of Trimethyltin on PC12 cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
The rat pheochromocytoma cell line PC12 cells were cultured in complete DMEM till 80% confluence, then placed at 5000 cells per squared cm. Cells were then plated in 24-well plates for cell viability assay and in T75 flasks for RNA isolation. Medium was replaced with serum-free fresh medium for 12 hours prior to TMT treatment.
Gene expression patterns were then analysed using Rat Expression Array 230A
Keywords: dose response, affymetrix chip
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| |
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| Overall design |
In this study we analize gene expression patterns in PC12 cells treated with Trimethyltin (TMT). We utilized control cells (untreated) and two different concentration (1 and 5 uM)
We used three biological replicates, for the three concentration tested, according to MIAME guidelines
(total 9 chips were used in this study).
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| |
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| Contributor(s) |
Bernardini C, Lattanzi W |
| Citation(s) |
17348866 |
| Submission date |
Jun 14, 2006 |
| Last update date |
Mar 03, 2017 |
| Contact name |
camilla bernardini |
| E-mail(s) |
camilla.bernardini@unicatt.it
|
| Phone |
+390630154711
|
| Organization name |
UCSC
|
| Department |
Human Anatomy
|
| Street address |
L.go F.Vito 1
|
| City |
Rome |
| ZIP/Postal code |
00186 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL341 |
[RAE230A] Affymetrix Rat Expression 230A Array |
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| Samples (9)
|
| GSM114191 |
Untreated cells pc12 contr_Signal |
| GSM114192 |
Low concentration treated cells pc12 1uM_Signal |
| GSM114193 |
High concentration treated cells pc12 5uM_Signal |
| GSM114194 |
Low concentration treated cells pc12 tmt 1uM_3_Signal |
| GSM114195 |
Low concentration treated cells pc12 tmt 1uM_4_Signal |
| GSM114196 |
High concentration treated cells pc12 tmt 5uM_5_Signal |
| GSM114197 |
High concentration treated cells pc12 tmt 5uM_6_Signal |
| GSM114198 |
Untreated cells pc12 tmt contr_1_Signal |
| GSM114199 |
Untreated cells pc12 tmt contr_2_Signal |
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| Relations |
| BioProject |
PRJNA95513 |
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