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| Status |
Public on Oct 01, 2013 |
| Title |
Quantification of gene expression from primate tissue samples |
| Organisms |
Macaca mulatta; Pan troglodytes; Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We have quantified gene expression in five tissues (brain, heart, kidney, liver and testis) from humans, chimpanzees and rhesus macaques using the Illumina NlaIII Digital Gene Expression (DGE) protocol. This dataset extends a previous microarray study by Khaitovich et al. (Khaitovich et al. 2005) with the rhesus macaque outgroup and complements other previously generated tissue transcriptome profiles from primates (Enard et al. 2002; Khaitovich et al. 2006; Somel et al. 2009; Babbitt et al. 2010; Blekhman et al. 2010; Wetterbom et al. 2010).
contributor:
Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, D-04103 Leipzig, Germany
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| Overall design |
Samples were obtained from brain (pre-frontal cortex), heart, kidney, liver, and testis tissues of male humans, chimpanzees and rhesus macaques. Illumina NlaIII DGE libraries for all samples were generated in tissue batches, randomizing species in library preparation and sequencing. Human samples originate from different, probably unrelated, individuals for each tissue. For chimpanzees and rhesus macaques the libraries for all tissues come from the same set of individuals and among these are individuals related at the half- and full-sibling level. Due to limited access to samples, the analysis could not be limited to individuals of similar age. Human individuals vary between 5 and 88 years of age, chimpanzees between 6 years and 35 years of age and rhesus macaques between 3 and 9 years of age.
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| Citation missing |
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| Submission date |
Sep 11, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Jay Shendure |
| Organization name |
University of Washington
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| Department |
Genome Sciences
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| Lab |
Shendure
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| Street address |
3720 15th Ave NE
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195-5065 |
| Country |
USA |
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| Platforms (6)
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| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
| GPL9160 |
Illumina Genome Analyzer II (Macaca mulatta) |
| GPL9378 |
Illumina Genome Analyzer II (Pan troglodytes) |
| GPL10547 |
Illumina Genome Analyzer (Pan troglodytes) |
| GPL13267 |
Illumina Genome Analyzer (Macaca mulatta) |
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| Samples (71)
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| Relations |
| BioProject |
PRJNA218879 |
| SRA |
SRP029889 |
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