|
| Status |
Public on Dec 05, 2013 |
| Title |
Polycomb-dependent H3K27me1 and H3K27me2 regulate active transcription and enhancer fidelity |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
These data include the genome wide location of different histone modifications by ChIP sequencing in mouse ES cells, and RNA Seq data generated from wild type and EED KO mouse ES cells and knocked down for unrelated protein and Setd2 protein.
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| |
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| Overall design |
ChIP-Seq: Immuno-precipitation of formaldehyde cross-linked chromatin prepared from wild type mouse E14 ES cells, wild type E36 ES cells, EED KO E36 ES cells, wild type Embryoid bodies (Ebs), EED KO Embryoid bodies (Ebs EED KO) using specific antibody against different histone modifications.
RNA-Seq: Total RNA extracted from wild type E36 ES cells, EED KO E36 ES cells, wild type E36 Embryoid bodies (Ebs), EED KO Embryoid bodies (Ebs EED KO), E14 Ctrl KD, E14 Setd2 KD.
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| |
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| Contributor(s) |
Ferrari K, Scelfo A, Jammula SG, Pasini D |
| Citation(s) |
24289921 |
| Submission date |
Sep 19, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Sri Ganesh Jammula |
| E-mail(s) |
sriganesh.jammula@ieo.eu
|
| Organization name |
IFOM-IEO
|
| Street address |
Via Adamello 16
|
| City |
Milan |
| ZIP/Postal code |
20139 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA219508 |
| SRA |
SRP030028 |
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