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| Status |
Public on Aug 28, 2006 |
| Title |
Hoxb1 gene expressing Hox-A1 protein: Altered transcriptional profile in rhombomere 4 at E10.5 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
This study analyzed mRNA profiles in rhombomere 4 of E10.5 mouse knock-in embryos expressing either normal endogenous Hox-B1 protein or the paralogous Hox-A1 protein from the Hoxb1 locus. The Hox-A1 protein was found to be detectably less efficacious than Hox-B1 in promoting neurogenesis in the basal plate of rhombomere 4 and its transcriptional profile shared several similarities with the Hoxb1 mutant.
Keywords: gene swap, knock-in, hindbrain development, rhombomere 4
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| Overall design |
GFP-positive cells were FACS-sorted from dissected hindbrains of entire litters of E10.5 mouse embryos expressing either normal endogenous Hox-B1 protein or the paralogous Hox-A1 protein from the Hoxb1 locus, either one tagged with IRES-tauGFP. Three independent biological replicates of each genotype were analyzed. Total RNA was isolated, amplified and hybridized to Affymetrix Mouse Genome 430 2.0 Arrays.
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| Contributor(s) |
Tvrdik P, Dunn DM, Weiss RB, Capecchi MR |
| Citation(s) |
16890163 |
| Submission date |
Jun 22, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Petr Tvrdik |
| E-mail(s) |
petr.tvrdik@genetics.utah.edu
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| Phone |
(801) 581-7097
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| Organization name |
University of Utah
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| Department |
Human Genetics
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| Lab |
Capecchi Lab
|
| Street address |
15 N 2030 E
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA96623 |
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