|
| Status |
Public on Apr 01, 2015 |
| Title |
H3K9 Trimethylation Silences Fas Expression to Confer Colon Carcinoma Immune Escape and 5-Fluorouracil Chemoresistance |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We have determined that verticillin A is a histone methyltransfease inhibitor that selectively inhibits human SUV39H1, SUV39H2, G9a and GLP to inhibit H3K9 methylation in human colon cancer cells. The objective here is to identify verticillin A target genes in human colon cancer cells.
|
| |
|
| Overall design |
5-FU-resistant human colon carcinoma cell lines LS411N-5FU-R was established from the metastatic human colon carcinoma cell line LS411N. LS411N-5FU-R cells were cultured in the absence or presence of verticillin A (50 nM) for 3 days and used for RNA lsolation. DNA microarray analysis was done in replicates in the Georgia Regents University Cancer Center Genomic Core Facility.
|
| |
|
| Contributor(s) |
Paschall AV, Liu K |
| Citation(s) |
26136424, 26309812 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| I01 BX001962 |
Function of Verticillin A in Suppression of MDSC and CRC Stem Cells |
CHARLIE NORWOOD VA MEDICAL CENTER |
LIU |
| R01 CA133085 |
Role of Epigenetic Repression of IRF8 in Tumor Progression/Metastasis: Role of IRF8 in Tumor Rejection and Suppression |
Georgia Regents Research Institute, Inc. |
LIU |
| R01 CA182518 |
Role of NF-kB in Fas-mediated Apoptosis and Tumor Suppression |
Georgia Regents Research Institute, Inc. |
LIU |
|
| Submission date |
Sep 28, 2013 |
| Last update date |
Jul 31, 2019 |
| Contact name |
Kebin Liu |
| E-mail(s) |
Kliu@augusta.edu
|
| Phone |
706-721-9483
|
| Organization name |
Medical College of Georgia
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Kebin Liu
|
| Street address |
1410 Laney Walker Blvd
|
| City |
Augusta |
| State/province |
GA |
| ZIP/Postal code |
30912 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA222251 |
Less...
More...