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| Status |
Public on Sep 01, 2006 |
| Title |
Gene Expression Biomarkers for Predicting Liver Tumors in Two-Year Rodent Bioassays |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Two-year rodent bioassays play a central role in evaluating both the carcinogenic potential of a chemical and generating quantitative information on the dose-response behavior for chemical risk assessments. The bioassays involved are expensive and time-consuming, requiring nearly lifetime exposures (two years) in mice and rats and costing $2 to $4 million per chemical. Since there are approximately 80,000 chemicals registered for commercial use in the United States and 2,000 more are added each year, applying animal bioassays to all chemicals of concern is clearly impossible. To efficiently and economically identify carcinogens prior to widespread use and human exposure, alternatives to the two-year rodent bioassay must be developed. In this study, animals were exposed for 13 weeks to two chemicals that were positive for liver tumors in the two-year rodent bioassay, two chemicals that were negative for liver tumors, and two vehicle controls. Gene expression analysis was performed on the livers of the animals to assess the potential for identifying gene expression biomarkers that can predict tumor formation in a two-year bioassay following a 13 week exposure.
Keywords: toxicology, chemical carcinogenesis, liver
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| Overall design |
Five week old female B6C3F1 mice were exposed for 13 weeks to the following treatments: 1) 1,5-Naphthalenediamine, CAS No. 2243-62-1, feed, 2000 ppm, positive liver carcinogen; 2) 2,3-Benzofuran, CAS No. 271-89-6, gavage, 240 mg/kg, positive liver carcinogen; 3) N-(1-naphthyl)ethylenediamine dihydrochloride, CAS No. 1465-25-4, feed, 2000 ppm, negative liver carcinogen; 4) Pentachloronitrobenzene, CAS No. 82-68-8, feed, 8187 ppm, negative liver carcinogen; 5) Feed control; 6) Corn oil gavage control. Feed animals were exposed 7 days/week and gavage animals were exposed 5 days/week (5 ml/kg). Microarray analysis was performed on the livers of three mice per treatment group.
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| Contributor(s) |
Thomas RS, Pluta LJ, Page TJ, Yang L |
| Citation(s) |
17114358 |
| Submission date |
Jun 22, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Russell Scott Thomas |
| E-mail(s) |
rthomas@thehamner.org
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| Phone |
919-558-1311
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| Fax |
919-558-1300
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| Organization name |
The Hamner Institutes for Health Sciences
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| Street address |
6 Davis Drive
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| City |
Research Triangle Park |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (18)
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| GSM115680 |
LiverTissue_GavageTreatment_2,3-Benzofuran_Animal10 |
| GSM115681 |
LiverTissue_FeedTreatment_N-(1-naphthyl)ethylenediamineDihydrochloride_Animal12 |
| GSM115682 |
LiverTissue_FeedTreatment_N-(1-naphthyl)ethylenediamineDihydrochloride_Animal14 |
| GSM115683 |
LiverTissue_FeedTreatment_N-(1-naphthyl)ethylenediamineDihydrochloride_Animal15 |
| GSM115684 |
LiverTissue_FeedTreatment_Pentachloronitrobenzene_Animal16 |
| GSM115685 |
LiverTissue_FeedTreatment_Pentachloronitrobenzene_Animal18 |
| GSM115686 |
LiverTissue_FeedTreatment_Pentachloronitrobenzene_Animal20 |
| GSM115687 |
LiverTissue_GavageTreatment_CornOil_Control_Animal22 |
| GSM115688 |
LiverTissue_GavageTreatment_CornOil_Control_Animal24 |
| GSM115689 |
LiverTissue_GavageTreatment_CornOil_Control_Animal25 |
| GSM115690 |
LiverTissue_FeedTreatment_Rodent Chow_Control_Animal27 |
| GSM115691 |
LiverTissue_FeedTreatment_Rodent Chow_Control_Animal28 |
| GSM115692 |
LiverTissue_FeedTreatment_Rodent Chow_Control_Animal29 |
| GSM115693 |
LiverTissue_FeedTreatment_1,5-Naphthalenediamine_Animal2 |
| GSM115694 |
LiverTissue_FeedTreatment_1,5-Naphthalenediamine_Animal4 |
| GSM115695 |
LiverTissue_FeedTreatment_1,5-Naphthalenediamine_Animal5 |
| GSM115696 |
LiverTissue_GavageTreatment_2,3-Benzofuran_Animal6 |
| GSM115697 |
LiverTissue_GavageTreatment_2,3-Benzofuran_Animal8 |
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| Relations |
| BioProject |
PRJNA96631 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE5128_RAW.tar |
104.6 Mb |
(http)(custom) |
TAR (of CEL) |
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