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| Status |
Public on Jan 18, 2016 |
| Title |
Helicobacter pylori-induced changes in miRNA expression in human gastric epithelial cells |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
H. pylori infection of human gastric epithelial cells (GEC) represses H,K-ATPase alpha subunit (HK-alpha) gene transcription through NF-Kappa B p50 homodimer binding to HK-alpha promoter. The bacterial cagA and slt gene products have been implicated in HK-alpha repression, which facilitates gastric H. pylori colonization and may underlie transient clinical hypochlorhydria. We hypothesized that H. pylori also regulates H,K-ATPase expression post-transcriptionally by micro-RNA interaction with HK-alpha mRNA. Our microarray experiment examined the effect of infection by wildtype H. pylori infection or a delta-cagA H. pylori mutant on miRNA expression of human gastric cells (AGS). Our results indicate that H. pylori infection up-regulates GEC miRNAs that target the HK-alpha 3' UTR and block translation, and that CagA activity is implicated in the miRNA up-regulation.
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| Overall design |
Three types of samples were generated for microarray analysis: 1) AGS cells infected with wildtype H. pylori; 2) AGS cells infected with cagA-deficient H. pylori; 3) a mock-infected sample, prepared as a control by combining total RNA from uninfected AGS cells with total RNA from wildtype H. pylori, with relative eukaryotic and prokaryotic RNA amounts matching those observed in infected samples, based on proportional ribosomal peak heights quantified by Bioanalyzer. Three experimental replicates were generated for each type of treatment.
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| Contributor(s) |
Smolka AJ, Barth JL |
| Citation missing |
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| Submission date |
Sep 30, 2013 |
| Last update date |
May 02, 2017 |
| Contact name |
Jeremy Barth |
| E-mail(s) |
barthj@musc.edu
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| Organization name |
MUSC
|
| Street address |
173 Ashley Ave, BSB 627
|
| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
29425-5080 |
| Country |
USA |
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| Platforms (1) |
| GPL14613 |
[miRNA-2] Affymetrix Multispecies miRNA-2 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA222375 |
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