|
| Status |
Public on Oct 22, 2013 |
| Title |
Comparison of microRNA Profiling Platforms (HTS) |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Global miRNA expression profiling of human malignancies is gaining popularity in both basic and clinically driven research. But to date, the majority of such analyses have used microarrays and quantitative real-time PCR. With the introduction of digital count technologies, such as next-generation sequencing (NGS) and the NanoString nCounter System, we have at our disposal, many more options. To make effective use of these different platforms, the strengths and pitfalls of several miRNA profiling technologies were assessed, including a microarray platform, NGS technologies and the NanoString nCounter System. These results were compared to gold-standard quantitative real-time PCR.
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| |
|
| Overall design |
Comparison of non-small cell lung cancer cell lines grown in vitro (n = 5) and in vivo (n = 5) as xenograft models.
|
| |
|
| Contributor(s) |
Tam S, de Borja R, Tsao M, McPherson JD |
| Citation(s) |
24445778 |
| Submission date |
Oct 21, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Shirley Tam |
| E-mail(s) |
shirley.tam@mail.utoronto.ca
|
| Organization name |
Ontario Institute for Cancer Research
|
| Department |
Genome Technologies
|
| Street address |
661 University Avenue, Suite 510, MaRS Building, West Tower
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A3 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (10)
|
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| This SubSeries is part of SuperSeries: |
| GSE51508 |
Comparison of microRNA Profiling Platforms |
|
| Relations |
| BioProject |
PRJNA223326 |
| SRA |
SRP031698 |
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