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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 24, 2013 |
| Title |
Treatment of prostate cancer cell lines DU-145 and LNCaP with zebularine |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Cultures of DU-145 cells and of LNCaP cells were treated for 216 hours with 100µM zebularine (SIGMA) in three independent biological experiments. Zebularine acts as a DNA methyltransferase (DNMT) inhibitor thereby upregulating genes that are inactivated by e.g. promotor hypermethylation. The experiment aimed to search for upregulated transcripts to provide new targets for biomarker development and therapeutic use.
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| Overall design |
Total RNA of untreated and treated (100µM zebularine) DU-145 cells (experiment HK_21_DU_145-HK_26_DU_145), and of untreated and treated (100µM zebularine) LNCaP cells (experiment HK_27_LNCaP-HK_32_LNCaP), were subjected to Affymetrix array analysis to detail the overall expression changes after treatment with a DNMT inhibitor. Treated cells showed no obvious signs of zebularine-induced cytotoxicity as revealed by XTT assays.
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| Contributor(s) |
Krause HG, Ikromov O |
| Citation |
Odiljon Ikromov, Imad Alkamal, AhmedMagheli, Nadine Ratert, Mauricio Sendeski, Kurt Miller, Hans Krause, and Carsten Kempkensteffen. Functional Epigenetic Analysis of Prostate Carcinoma: A Role for Seryl-tRNA Synthetase? Hindawi Publishing Corporation, Journal of Biomarkers Volume 2014, Article ID 362164, 10 pages. http://dx.doi.org/10.1155/2014/362164
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| Submission date |
Oct 23, 2013 |
| Last update date |
Jan 25, 2019 |
| Contact name |
Hans G Krause |
| E-mail(s) |
hans.krause@charite.de
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| Organization name |
Charité - Universitätsmedizin Berlin
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| Department |
Klinik für Urologie
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| Street address |
Charitéplatz 1
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| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
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| Platforms (1) |
| GPL10739 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [probe set (exon) version] |
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| Samples (12)
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| GSM1249704 |
Untreated control, DU-145, biological rep 1 |
| GSM1249705 |
100µM zebularine, DU-145, biological rep 1 |
| GSM1249706 |
Untreated control, DU-145, biological rep 2 |
| GSM1249707 |
100µM zebularine, DU-145, biological rep 2 |
| GSM1249708 |
Untreated control, DU-145, biological rep 3 |
| GSM1249709 |
100µM zebularine, DU-145, biological rep 3 |
| GSM1249710 |
Untreated control, LNCaP, biological rep 1 |
| GSM1249711 |
100µM zebularine, LNCaP, biological rep 1 |
| GSM1249712 |
Untreated control, LNCaP, biological rep 2 |
| GSM1249713 |
100µM zebularine, LNCaP, biological rep 2 |
| GSM1249714 |
Untreated control, LNCaP, biological rep 3 |
| GSM1249715 |
100µM zebularine, LNCaP, biological rep 3 |
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| Relations |
| BioProject |
PRJNA224117 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE51629_RAW.tar |
49.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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