|
| Status |
Public on Jan 31, 2014 |
| Title |
Gene expression analysis upon stable SmcHD1 Knock Down |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To study the role of SmcHD1 in regulating gene expression, we have stably knocked down SmcHD1 in HEK293 cells and looked at change in gene expression.
shRNA3 and shRNA4 were used to target the expression of the SmcHD1 gene (Genbank Accession number NM_015295). The protein levels of SmcHD1 were knocked down in excess of 90% normal levels in 293 cells. shRNA NC5 a scrambled control shRNA and did not change SmcHD1 protein levels in 293 cells.
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| |
|
| Overall design |
Agilent single color 28004 was used on 9 Samples.
|
| |
|
| Contributor(s) |
Prefontaine GG, Massah S, Haegert A |
| Citation(s) |
24818964 |
| Submission date |
Nov 04, 2013 |
| Last update date |
Oct 11, 2016 |
| Contact name |
Gratien G. Prefontaine |
| E-mail(s) |
gprefontaine@gmail.com
|
| Phone |
7783853453
|
| Organization name |
Simon Fraser University
|
| Department |
Faculty of Health Sciences
|
| Lab |
Prefontaine
|
| Street address |
8888 University Drive
|
| City |
Burnaby |
| State/province |
British Columbia |
| ZIP/Postal code |
V5A1S6 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA226720 |
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