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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 03, 2008 |
| Title |
Profiling of CD4+ T cells responding to transient or persistent antigen presented by dendritic cells in vivo |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
These experiments were done to compare the gene expression profiles in CD4+ T cells responding to antigen presented by dendritic cells transiently or persistently. Some treatments include the activation of the dendritic cells by CD40 engagement.
Keywords: immune response
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| Overall design |
2-4x106 lymph node cells from AND TCR-transgenic animals that expressed the congenic marker CD90.1 were transferred into double-transgenic (Ii-rTA/TIM) recipient animals that expressed the corresponding peptide from moth cytochrome c under a doxycycline-controllable promoter and were FACS-sorted 60 hours later. Recipients expressed the antigen not at all (“ctrl”), transiently (“short”) or throughout the experiment (“long”) due to different doxycycline treatment protocols. A second variable tested was the activation status of the dendritic cells: recipients were either injected by the stimulatory antibody FGK45.5 targeting CD40 (“FGK”) or PBS (“PBS”).
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| Contributor(s) |
Obst R, van Santen H, Melamed R, Benoist C, Mathis D |
| Citation(s) |
17881563 |
| Submission date |
Jul 06, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
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| Phone |
617-432-7747
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| Organization name |
Harvard Medical School
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| Department |
Microbiology and Immunobiology
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| Lab |
CBDM
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| Street address |
77 Avenue Louis Pasteur
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (16)
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| GSM118668 |
AND T cells-no dox, FGK-rep1 |
| GSM118669 |
AND T cells-no dox, FGK-rep2 |
| GSM118671 |
AND T cells-short dox, PBS-rep1 |
| GSM118673 |
AND T cells-short dox, PBS-rep2 |
| GSM118674 |
AND T cells-short dox, PBS-rep3 |
| GSM118677 |
AND T cells-short dox, FGK-rep1 |
| GSM118679 |
AND T cells-short dox, FGK-rep2 |
| GSM118681 |
AND T cells-long dox, PBS-rep1 |
| GSM118682 |
AND T cells-long dox, PBS-rep2 |
| GSM118684 |
AND T cells-long dox, PBS-rep3 |
| GSM118686 |
AND T cells-long dox, FGK-rep1 |
| GSM118687 |
AND T cells-long dox, FGK-rep2 |
| GSM118689 |
AND T cells-long dox, FGK-rep3 |
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| Relations |
| BioProject |
PRJNA96463 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE5245_RAW.tar |
89.4 Mb |
(http)(custom) |
TAR (of CEL) |
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