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| Status |
Public on Feb 27, 2014 |
| Title |
Novel primate miRNAs co-evolved with ancient target genes in germinal zone specific expression patterns [RISC trap immunoprecipitation] |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
Major non primate-primate differences in corticogenesis include the dimensions, precursor lineages and developmental timing of the germinal zones (GZ). microRNAs (miRNAs) of laser dissected GZ compartments and cortical plate (CP) from embryonic E80 macaque visual cortex were deep sequenced. The CP and the GZ including Ventricular Zone (VZ), outer and inner subcompartments of the Outer SubVentricular Zone (OSVZ) in area 17 displayed unique miRNA profiles. miRNAs present in primate, but absent in rodent, contributed disproportionately to the differential expression between GZ sub-regions. Prominent among the validated targets of these miRNAs were cell-cycle and neurogenesis regulators. Co-evolution between the emergent miRNAs and their targets suggested that novel miRNAs became integrated into ancient gene circuitry to exert additional control over proliferation. We conclude that multiple cell-cycle regulatory events contribute to the emergence of primate-specific cortical features, including the OSVZ, generated enlarged supragranular layers, largely responsible for the increased primate cortex computational abilities.
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| Overall design |
target mRNAs for selected miRNAs were detected with RISC trap immunoprecipitation
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| Contributor(s) |
Arcila ML, Betizeau M, Cambronne XA, Guzman E, Doerflinger N, Bouhallier F, Zhou H, Wu B, Huissoud C, Rani N, Bassett DS, Borello U, Goodman RH, Dehay C, Kosik KS |
| Citation(s) |
24583023 |
| Submission date |
Nov 21, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Kenneth S. Kosik |
| Organization name |
UCSB
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| Department |
NRI
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| Lab |
Kosik
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| Street address |
UC Santa Barbara
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| City |
Santa Barbara |
| State/province |
California |
| ZIP/Postal code |
93106 |
| Country |
USA |
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| Platforms (2) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
| GPL13393 |
AB SOLiD 4 System (Homo sapiens) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE52608 |
Novel primate miRNAs co-evolved with ancient target genes in germinal zone specific expression patterns |
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| Relations |
| BioProject |
PRJNA229466 |
| SRA |
SRP033233 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52607_refseq.txt.gz |
618.7 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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