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| Status |
Public on Jul 21, 2014 |
| Title |
Replication profile from total DNA |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
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| Summary |
DNA duplication is intimately connected to setting up post-replicative chromosome structures and events, but molecular details of this coordination are not well understood. A striking example occurs during yeast meiosis, where replication locally influences timing of the DNA double-strand breaks (DSBs) that initiate recombination. We show here that replication-DSB coordination is eliminated by overexpressing Dbf4-dependent Cdc7 kinase (DDK) or removing Tof1 or Csm3, components of the replication fork protection complex (FPC). DDK physically associates with Tof1, and Tof1 is dispensable for replication-DSB coordination if DDK is artificially tethered to replisomes. Furthermore, DDK phosphorylation of the DSB-promoting factor Mer2 is locally coordinated with replication, dependent on Tof1. These findings indicate that DDK recruited by FPC to replisomes phosphorylates chromatin-bound Mer2 in the wake of the replication fork, thus synchronizing replication with an early prerequisite for DSB formation. This may be a general mechanism to ensure spatial and temporal coordination of replication with other chromosomal processes.
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| Overall design |
Forty-eight samples total: 8 time points from WT ARS+, WT ars∆, DDK OP ARS+, DDK OP ars∆,tof1∆ ARS+,tof1∆ ars∆ strains
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| Contributor(s) |
Murakami H, Keeney S |
| Citation(s) |
25126790 |
| Submission date |
Dec 04, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Bic MSKCC |
| Organization name |
Memorial SLoan-Kettering Cancer Center
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| Street address |
1275 York Ave.
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA230630 |
| SRA |
SRP033509 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52987_DDK_A+.txt.gz |
257.1 Kb |
(ftp)(http) |
TXT |
| GSE52987_DDK_a-.txt.gz |
256.6 Kb |
(ftp)(http) |
TXT |
| GSE52987_WT_A+.txt.gz |
256.0 Kb |
(ftp)(http) |
TXT |
| GSE52987_WT_a-.txt.gz |
257.3 Kb |
(ftp)(http) |
TXT |
| GSE52987_tof1_A+.txt.gz |
256.8 Kb |
(ftp)(http) |
TXT |
| GSE52987_tof1_a-.txt.gz |
257.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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