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| Status |
Public on Mar 01, 2016 |
| Title |
Genome-wide screening for ELF4-binding sites. |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ELF4 (also known as MEF) is a member of the ETS family of transcriptional factors. While an oncogenic role has been demonstrated for ELF4 mainly in hematopoietic malignancies, its definitive function in human carcinogenesis is not clear yet. Here we demonstrate that a wide array of human tumors carry somatic, loss-of-function mutations in ELF4 for its transcriptional activity, and restoring its function exerts anti-proliferative effects. To further explore the tumor suppressive function of ELF4, its binding sites were searched among the human genome with the use of chromatin immunoprecipitation coupled with sequencing (ChIP-seq).
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| |
|
| Overall design |
Examination of binding site for FLAG-immunoprecipitated mock, ELF4 (WT) and ELF4 (L211M) with each input sample as a control.
|
| |
|
| Contributor(s) |
Ando M, Ueno T, Mano H |
| Citation(s) |
26921333 |
| Submission date |
Dec 16, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Mizuo Ando |
| E-mail(s) |
ando.hnc@gmail.com
|
| Phone |
03-3815-5411
|
| Organization name |
The University of Tokyo
|
| Department |
Otolaryngology-Head and Neck Surgery
|
| Street address |
7-3-1 Hongo
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA231834 |
| SRA |
SRP034521 |
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