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| Status |
Public on Dec 31, 2015 |
| Title |
Gene expression profiling of primary human hepatocytes after berberine chloride treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Berberine, an isoquinoline alkaloid isolated from many medicinal herbs such as Coptis chinensis, has a wide range of pharmacological effects. Since xenobiotic drug-induced micoRNAs have recently emerged as key regulators in guiding their pharmacological effects and toxicity, we were interested in whether or not micoRNA expression was differentially altered by berberine treatment in liver. Here, we used miRNA microarray to analyze microRNA expression profiles of primary human hepatocytes after berberine chloride treatment or 0.08% DMSO as control.
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| Overall design |
Comparing gene expression profiles of 40 M berberine-treated primary human hepatocytes to those of control cells sampled after 2, 4, or 8 hours treatment. A 50 mM stock solution of Berberine chloride was prepared in DMSO. Cells were treated with 40 M berberine chloride or 0.08% DMSO as control.
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| Contributor(s) |
Lo T, Chen S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 17, 2013 |
| Last update date |
Oct 11, 2016 |
| Contact name |
Ting-fang Lo |
| E-mail(s) |
r96b47204@ntu.edu.tw
|
| Phone |
+886-939964794
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| Organization name |
Academia Sinica
|
| Department |
Institute of Biological Chemistry
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| Street address |
128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan
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| City |
Taipei |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE53416 |
MicroRNA and gene expression profiling of primary human hepatocytes after berberine chloride treatment |
|
| Relations |
| BioProject |
PRJNA231939 |
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