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| Status |
Public on Dec 18, 2013 |
| Title |
SNP data from human fibroblasts and induced Pluripotent Stem cells from Parkinson's Disease patients |
| Organism |
Homo sapiens |
| Experiment type |
SNP genotyping by SNP array
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| Summary |
We have generated human induced Pluripotent Stem cells (hiPSc) from Parkinson's Disease patients, using retrovirus-mediated delivery of reprogramming factors. hiPSc lines have been screened using SNP array to assess chromosomal stability (alongside the fibroblast lines from which they derived), and validation of the pluripotency of the hiPSc lines is provided by Pluritest assessment of transcriptome datasets, prior to differentiation to dopaminergic neuronal clutures and downstream functional assays.
Fernandes H.J.R., Hartfield E.M., Badger J., Christian H. C., Emmanoulidou E., Vowles J., Evetts S., Vekrellis K., Talbot K., Hu M.T., James W., Cowley S.A., and Wade-Martins, R. Heterozygous glucocerebrosidase mutations in Parkinson's increase autophagic demand, but decrease capacity, in induced pluripotent stem cell-derived dopaminergic neuronal cultures. submitted for publication
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| Overall design |
human iPSc lines were derived from human dermal fibroblasts from 2 Parkinson's Disease patients with heterozygous glucocerebrosidase mutations (GBA N370S) mutations, and 2 idiopathic Parkinson's Disease patients. SNP datasets from the 2 control individuals used in this study have been published previously [PMID 23951090 GSE45472; PMID:24586273 GSE43904]
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| Contributor(s) |
Fernandes HJ, Hartfield EM, Badger J, Christian HC, Emmanoulidou E, Vowles J, Evetts S, Vekrellis K, Talbot K, Hu MT, James W, Cowley SA, Wade-Martins R |
| Citation(s) |
26905200, 27475716 |
| Submission date |
Dec 17, 2013 |
| Last update date |
Dec 18, 2017 |
| Contact name |
Sally A Cowley |
| E-mail(s) |
sally.cowley@path.ox.ac.uk
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| Phone |
+44 (0) 1865 275600
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| Organization name |
University of Oxford
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| Department |
Sir William Dunn School of Pathology
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| Lab |
James Martin Stem Cell Facility
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| Street address |
South Parks Road
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| City |
Oxford |
| ZIP/Postal code |
OX1 3RE |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL13829 |
Illumina HumanCytoSNP-12 v2.1 BeadChip |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE53426 |
Gene expression and genotype data from human fibroblasts and induced Pluripotent Stem cells from Parkinson's Disease patients |
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| Relations |
| BioProject |
PRJNA231957 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE53425_non_normalized.txt.gz |
36.2 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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