|
| Status |
Public on Dec 20, 2013 |
| Title |
Genome-wide promoter methylation analysis associated with malignant melanoma progression |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
|
| Summary |
Epigenetic alterations play significant roles in the melanoma tumorigenesis and malignant progression. We profiled genome-wide promoter DNA methylation patterns of melanoma cells deribed from primary lesions of Radial Growrth phase (RGP) and Vertical Growth Phase (VGP), metastatic lesions, and primary normal melanocytes by interrogating 14,495 genes using Illumina bead chip technology. By comparative analysis of the promoter methylation profiles, we identified epigenetically silenced gene signatures that potentially associated with malignant melanoma progression.
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| Overall design |
Bisulphite converted genomic DNA from a group of melanoma cells representing pathologic stages of melanoma progression (3 cell lines derived from RGP melanoma lesions, 4 cell lines derived from VGP lesions, and 3 melastatic melanomas) and normal human primary melanocytes isolated from lightly pigmented adult skin were hybridized to Illumina's Infinium HumanMethylation27 BeadChips
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| Contributor(s) |
Ryu B, Kim H, Hwang S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 19, 2013 |
| Last update date |
Jan 02, 2015 |
| Contact name |
Byungwoo Ryu |
| E-mail(s) |
ryub@bu.edu
|
| Organization name |
Boston University
|
| Department |
Dermatology
|
| Street address |
609 Albany Street
|
| City |
Boston |
| State/province |
MA - Massachusetts |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL8490 |
Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2) |
|
| Samples (12)
|
| GSM1295307 |
genomic DNA from cultured human primay melanocytes [HPM1] |
| GSM1295308 |
genomic DNA from cultured human melanoma cells [HPM2] |
| GSM1295309 |
genomic DNA from cultured melanoma cells [WM35] |
| GSM1295310 |
genomic DNA from cultured melanoma cells [Sbcl2] |
| GSM1295311 |
genomic DNA from cultured melanoma cells [WM1552C] |
| GSM1295312 |
genomic DNA from cultured melanoma cells [WM902B] |
| GSM1295313 |
genomic DNA from cultured melanoma cells [WM278] |
| GSM1295314 |
genomic DNA from cultured melanoma cells [WM983A] |
| GSM1295315 |
genomic DNA from cultured melanoma cells [WM793] |
| GSM1295316 |
genomic DNA from cultured melanoma cells [WM852] |
| GSM1295317 |
genomic DNA from cultured melanoma cells [WM983B] |
| GSM1295318 |
genomic DNA from cultured melanoma cells [1205Lu] |
|
| Relations |
| BioProject |
PRJNA232260 |
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