|
| Status |
Public on Dec 17, 2014 |
| Title |
RNF17 referees ping-pong in mouse testes [RNAseq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In mammals, piRNA populations are dynamic throughout male germ cell development. Embryonic piRNAs consist of both primary and secondary species and are mainly directed toward transposons. In meiotic cells, however, the piRNA population is transposon-poor and restricted to primary piRNAs derived from pachytene piRNA clusters. The mechanism controlling which piRNAs are present at each developmental stage is poorly understood. Here we show that RNF17 shapes adult meiotic piRNA content by suppressing the production of secondary piRNAs. In the absence of RNF17, ping-pong (secondary amplification) occurs inappropriately in meiotic cells – aberrantly targeting protein-coding genes and lncRNAs. Our data indicate that RNF17 comprises one component of a “refereeing” mechanism that prevents deleterious activity of the meiotic piRNA pathway by ensuring the selective loading of PIWI proteins with products of meiotic piRNA clusters.
|
| |
|
| Overall design |
Examination of transcriptom in heterozygous and homozygous RNF17 adult testes and RNF17 immunoprecipitates
|
| |
|
| Contributor(s) |
Wasik KA, Tam O, Hao Y, Falciatori I, Hammell M, Vagin VV, Hannon GJ |
| Citation missing |
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| Submission date |
Jan 08, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Vasily Vagin |
| E-mail(s) |
vagin@cshl.edu
|
| Organization name |
Cold Spring Harbor
|
| Lab |
Hannon's
|
| Street address |
1 Bungtown Rd
|
| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
|
| Samples (8)
|
| GSM1303467 |
het RNF17 6 week old adult testis rep1 RNAseq |
| GSM1303468 |
hom RNF17 6 week old adult testis rep1 RNAseq |
| GSM1303469 |
het RNF17 6 week old adult testis rep2 RNAseq |
|
| This SubSeries is part of SuperSeries: |
| GSE53919 |
RNF17 blocks promiscuous activity of PIWI proteins in mouse testes |
|
| Relations |
| BioProject |
PRJNA233959 |
| SRA |
SRP035245 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE53913_Differentially_expressed_genes_in_hom_RNF17_testes.txt.gz |
32.0 Kb |
(ftp)(http) |
TXT |
| GSE53913_Genic_reads_in_heterozygouse_RNF17_lysate_and_RNF17_IP.txt.gz |
165.7 Kb |
(ftp)(http) |
TXT |
| GSE53913_Genic_reads_in_hom_and_heterozygouse_RNF17_adult_testes.txt.gz |
174.6 Kb |
(ftp)(http) |
TXT |
| GSE53913_Genic_reads_in_homozygouse_RNF17_lysate_and_RNF17_IP.txt.gz |
169.4 Kb |
(ftp)(http) |
TXT |
| GSE53913_Multiple_transposon_reads_in_heterozygouse_RNF17_lysate_and_RNF17_IP.txt.gz |
11.6 Kb |
(ftp)(http) |
TXT |
| GSE53913_Multiple_transposon_reads_in_hom_and_heterozygouse_RNF17_adult_testes.txt.gz |
11.7 Kb |
(ftp)(http) |
TXT |
| GSE53913_Multiple_transposon_reads_in_homozygouse_RNF17_lysate_and_RNF17_IP.txt.gz |
10.0 Kb |
(ftp)(http) |
TXT |
| GSE53913_Unique_transposon_reads_in_heterozygouse_RNF17_lysate_and_RNF17_IP.txt.gz |
14.4 Kb |
(ftp)(http) |
TXT |
| GSE53913_Unique_transposon_reads_in_hom_and_hetrozygouse_RNF17_adult_testes.txt.gz |
15.1 Kb |
(ftp)(http) |
TXT |
| GSE53913_Unique_transposon_reads_in_homozygouse_RNF17_lysate_and_RNF17_IP.txt.gz |
14.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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