|
| Status |
Public on Jan 16, 2014 |
| Title |
Microarray analysis of Nurr1 and Foxa2 synergisitic effect for DA neuron induction |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of dopaminergic neuronal gene expression changes by Nurr1 and/or Foxa2 overexpression. Result provides that Foxa2 potentiates Nurr1-induced DA neuronal phenotype gene expression.
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| |
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| Overall design |
To identify the syergism of Nurr1 and Foxa2 for developing DA neural precursors, neural precusor cells (NPCs) isolated from embryonic brain were treated control, Nurr1, Foxa2 and Nurr1-Foxa2 retrovirus. After treatment of retroviruses, NPCs were cultrued in N2 media withdrawn mitogen (bFGF, EGF) for differetiation of DA neuron. Total RNA was obtained from NPCs in differentiation day 2.
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| |
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| Contributor(s) |
Yi S, He X, Lee S |
| Citation(s) |
24496614 |
| Submission date |
Jan 15, 2014 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Sang-Hun Lee |
| E-mail(s) |
leesh@hanyang.ac.kr
|
| Phone |
82-2-2220-0625
|
| Organization name |
College of medicine, Hanyang University
|
| Department |
biochemistry and molecular biology
|
| Street address |
222 Wangsimni-ro, Seongdong-gu
|
| City |
Seoul |
| ZIP/Postal code |
133-791 |
| Country |
South Korea |
| |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA235158 |
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