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Series GSE54514 Query DataSets for GSE54514
Status Public on Feb 04, 2014
Title Whole blood transcriptome of survivors and nonsurvivors of sepsis
Organism Homo sapiens
Experiment type Expression profiling by array
Summary There is currently no reliable tool available to measure immune dysfunction in septic patients in the clinical setting. This proof-of-concept study assesses the potential of gene expression profiling of whole blood as a tool to monitor immune dysfunction in critically ill septic patients. Whole blood samples were collected daily for up to 5 days from patients admitted to the intensive care unit with sepsis. RNA isolated from whole blood samples was assayed on Illumina HT-12 gene expression microarrays consisting of 48,804 probes. Microarray analysis identified 3677 genes as differentially expressed across 5 days between septic patients and healthy controls. Of the 3677 genes, biological pathway analysis identified 86 genes significantly down-regulated in the sepsis patients were present in pathways relating to immune response. These 86 genes correspond to known immune pathways implicated in sepsis including lymphocyte depletion, reduced T lymphocyte activation and deficient antigen presentation. Furthermore, expression levels of these genes correlated with clinical severity, with a significantly greater degree of down-regulation found in non-survivors compared to survivors. The results show that whole blood gene-expression analysis can capture systemic immune dysfunctions in septic patients. Our study provides an experimental basis to support further study on the use of a gene expression based assay, to assess immunosuppression and guide immunotherapy in future clinical trials.
 
Overall design Daily PAXgene samples for up to 5 days for sepsis survivors (n=26), sepsis nonsurvivors (n=9), and healthy controls (n=18).
 
Contributor(s) Parnell GP, Tang BM
Citation(s) 23807251
Submission date Jan 29, 2014
Last update date Aug 16, 2018
Contact name Grant Parnell
E-mail(s) gpar3109@uni.sydney.edu.au
Organization name University of Sydney
Department Nepean Clinical School
Lab Intensive Care
Street address Nepean Hospital
City Kingswood
State/province NSW
ZIP/Postal code 2747
Country Australia
 
Platforms (1)
GPL6947 Illumina HumanHT-12 V3.0 expression beadchip
Samples (163)
GSM1317896 PAXgene whole blood, Control, Day_1, ID=1
GSM1317897 PAXgene whole blood, Control, Day_1, ID=2
GSM1317898 PAXgene whole blood, Control, Day_1, ID=3
Relations
BioProject PRJNA237335

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54514_RAW.tar 6.2 Mb (http)(custom) TAR
GSE54514_non-normalized.txt.gz 31.7 Mb (ftp)(http) TXT
Processed data included within Sample table
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