|
| Status |
Public on Sep 15, 2014 |
| Title |
RNA-seq from control and macroH2A1-depleted IMR90 primary human lung fibroblasts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The histone variant macroH2A1 and the poly(ADP-ribose) polymerase PARP-1 both regulate gene transcription by modulating chromatin structure and function. Of the two macroH2A1 splice variants, macroH2A1.1 and macroH2A1.2, the former is often suppressed in cancer and has the unique ability to interact with poly(ADP-ribose). Using ChIP-seq in primary lung fibroblasts, we demonstrate that macroH2A1 is incorporated into either of two spatially and functionally distinct types of chromatin; the first is marked by H3 K27 trimethylation, while the second contains a set of nine histone acetylations. MacroH2A1-regulated genes are involved in cancer progression are specifically found in macroH2A1-containing acetylated chromatin. Through the recruitment of PARP-1, macroH2A1.1 promotes the acetylation of H2B K12 and K120 which plays a key role in the regulation of macroH2A1 target genes in primary cells. The macroH2A1/PARP-1 pathway regulating H2B K12 and K120 acetylation is disrupted in cancer cells, in part, explaining macroH2A1’s role in cancer suppression.
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| |
|
| Overall design |
Three biological replicates of RNA-seq from cells expressing shRNA directed against macroH2A1 or luciferase as a control
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| |
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| Contributor(s) |
Gamble MJ, Chen H |
| Citation(s) |
25306110 |
| Submission date |
Feb 10, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Matthew Jon Gamble |
| E-mail(s) |
matthew.gamble@einstein.yu.edu
|
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Molecular Pharmacology
|
| Lab |
Gamble Lab
|
| Street address |
1300 Morris Park Ave / Golding 203
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (6)
|
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| This SubSeries is part of SuperSeries: |
| GSE54847 |
Exploring the role of macroH2A1 in transcription regulation in IMR90 primary human lung fibroblasts with RNA-seq and ChIP-seq |
|
| Relations |
| BioProject |
PRJNA237809 |
| SRA |
SRP037550 |
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