|
| Status |
Public on Apr 03, 2014 |
| Title |
Identification of new regulators of the three dimensional Polycomb organization by a microscopy-based genome-wide RNAi screen |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Polycomb group (PcG) proteins dynamically define cellular identities through epigenetic repression of key developmental genes. PcG target gene repression can be stabilized through the interaction in the nucleus at PcG foci. Here, we report the results of a high-resolution microscopy genome-wide RNAi screen that identifies 129 genes that regulate the nuclear organization of Pc foci. Candidate genes include PcG components and chromatin factors, as well as many novel protein-modifying enzymes, including components of the SUMOylation pathway. In the absence of SUMO Pc foci coagulate into larger aggregates. Conversely, loss of function of the SUMO peptidase velo disperses Pc foci. Moreover, SUMO and velo colocalize with PcG proteins at PREs and Pc SUMOylation affects its chromatin targeting, suggesting that the dynamic regulation of Pc SUMOylation regulates PcG-mediated silencing by modulating the kinetics of Pc binding to chromatin as well as its ability to form Polycomb foci.
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| |
|
| Overall design |
ChIP-Seq mapping of Polycomb (PC), SUMO and Velo on Drosophila Melanogaster
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| |
|
| Contributor(s) |
Gonzalez I, Mateos-Langerak J, Thomas A, Cheutin T, Cavalli G |
| Citation(s) |
24703951 |
| Submission date |
Feb 24, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Giacomo Cavalli |
| E-mail(s) |
giacomo.cavalli@igh.cnrs.fr
|
| Organization name |
CNRS
|
| Lab |
INSTITUTE of HUMAN GENETICS
|
| Street address |
IGH - CNRS, 141, rue de la Cardonille
|
| City |
Montpellier |
| ZIP/Postal code |
34396 Cedex5 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
|
| Samples (7)
|
| GSM1333823 |
ChIP-Seq Analysis of SUMO in S2 cells, first replicate |
| GSM1333824 |
ChIP-Seq Analysis of SUMO in S2 cells, second replicate |
| GSM1333825 |
ChIP-Seq Analysis of Velo in S2 cells, first replicate |
| GSM1333826 |
ChIP-Seq Analysis of Velo in S2 cells, second replicate |
| GSM1333827 |
ChIP-Seq Analysis of PC in S2 cells, first replicate |
| GSM1333828 |
ChIP-Seq Analysis of PC in S2 cells, second replicate |
| GSM1333829 |
ChIP-Seq input in S2 cells |
|
| Relations |
| BioProject |
PRJNA239247 |
| SRA |
SRP038890 |
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