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| Status |
Public on Mar 04, 2014 |
| Title |
miRNA expression in fast and slow skeletal muscle of wild type and SOD1-G93A mice |
| Platform organism |
synthetic construct |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Amyotrophic lateral sclerosis (ALS) is a lethal motor neuron disease that progressively debilitates neuronal cells that control voluntary muscle activity. In a mouse model of ALS that expresses mutated human superoxide dismutase 1 (SOD1-G93A) skeletal muscle is one of the tissues affected early by mutant SOD1 toxicity. Fast-twitch and slow-twitch muscles are differentially affected in ALS patients and in the SOD1-G93A model, fast-twitch muscles being more vulnerable.
We used miRNA microarrays to investigate miRNA alterations in fast-twitch (EDL) and slow-twitch (soleus) skeletal muscles of symptomatic SOD1-G93A animals and their age-matched wild type littermates.
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| Overall design |
At age of 90 days RNA was extracted from extensor digitorum longus (EDL) and soleus (SOL) muscles of male SOD1-G93A animals and their age-matched wild type male littermates. RNA was hybridized on Affymetrix Multispecies miRNA-2_0 Array.
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| Contributor(s) |
Toivonen JM, Manzano R, Olivan S, Zaragoza P, Garcia-Redondo A, Osta R |
| Citation(s) |
24586506 |
| Submission date |
Mar 03, 2014 |
| Last update date |
May 02, 2017 |
| Contact name |
Janne M Toivonen |
| E-mail(s) |
toivonen@unizar.es
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| Organization name |
Universidad de Zaragoza
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| Department |
Departamento de Anatomía, Embriología y Genética Animal
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| Lab |
Laboratorio de Genética Bioquímica (LAGENBIO-I3A)
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| Street address |
Miguel servet 177
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| City |
Zaragoza |
| ZIP/Postal code |
50013 |
| Country |
Spain |
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| Platforms (1) |
| GPL14613 |
[miRNA-2] Affymetrix Multispecies miRNA-2 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA239806 |
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