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| Status |
Public on Apr 09, 2015 |
| Title |
Transcriptome Analysis Predicts Clinical Outcome and Sensitivity to Anticancer Drugs of patients with a Pancreatic Adenocarcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
A major impediment to the effective treatment of patients with PDAC (Pancreatic Ductal Adenocarcinoma) is the molecular heterogeneity of the disease, which is reflected in an equally diverse pattern of clinical responses to therapy. We developed an efficient strategy in which PDAC samples from 17 consecutively patients were obtained by EUS-FNA or surgery, their cells maintained as a primary culture and tumors as breathing tumors by xenografting in immunosuppressed mice. For these patients a clinical follow up was obtained. On the breathing tumors we studied the RNA expression profile by an Affymetrix approach. We observed a significant heterogeneity in their RNA expression profile, however, the transcriptome was able to discriminate patients with long- or short-time survival which correspond to moderately- or poorly-differentiated PDAC tumors respectively. Cells allowed us the possibility to analyze their relative sensitivity to several anticancer drugs in vitro by developing a chimiogram, like an antibiogram for microorganisms, with several anticancer drugs for obtaining an individual profile of drug sensitivity and as expected, the response was patient-dependent. Interestingly, using this approach, we also found that the transcriptome analysis could predict the sensitivity to some anticancer drugs of patients with a PDAC. In conclusion, using this approach, we found that the transcriptome analysis could predict the sensitivity to some anticancer drugs and the clinical outcome of patients with a PDAC.
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| Overall design |
PDAC samples from 17 consecutively patients were obtained by Endoscopic Ultrasound-Guided Fine-Needle Aspiration (EUS-FNA) or surgery. Consents of informed patients were collected, and the ethics review board of the three centers approved the study. All samples were anonymized and obtained in accordance with institutional review boards. EUS-FNA cells were maintained as a primary culture and tumors as breathing tumors by xenografting in immunosuppressed mice. We characterized 17 PDAC-derived xenografts by duplicate. Total RNA was extracted and hybridized on Human Gene 2.0 (Genechip, Affymetrix) as described previously (Hamidi et al. JCI 122: 2092-2103, 2012).
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| Contributor(s) |
Duconseil P, Gilabert M, Gayet O, Loncle C, Moutardier V, Turrini O, Calvo E, Ewald J, Giovannini M, Gasmi M, Bories E, Barthet M, Ouaissi M, Goncalves A, Raoul J, Secq V, Garcia S, Viens P, Iovanna J, Dusetti N |
| Citation(s) |
25765988, 28275007, 29844366, 29161242 |
| Submission date |
Mar 03, 2014 |
| Last update date |
Jul 08, 2019 |
| Contact name |
Ezequiel L Calvo |
| E-mail(s) |
cezequiel@yahoo.com
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| Organization name |
CRCHUL
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| Department |
Molecular Endocrinilogy
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| Lab |
Microarrays
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| Street address |
2705 Boul. Laurier
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| City |
Quebec |
| State/province |
Quebec |
| ZIP/Postal code |
G1V 4G2 |
| Country |
Canada |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (34)
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| GSM1338416 |
PDAC xenograft from patient 01.001-rep 1 |
| GSM1338417 |
PDAC xenograft from patient 01.001-rep 2 |
| GSM1338418 |
PDAC xenograft from patient H-Nor-rep 1 |
| GSM1338419 |
PDAC xenograft from patient H-Nor-rep 2 |
| GSM1338420 |
PDAC xenograft from patient HN03-rep 1 |
| GSM1338421 |
PDAC xenograft from patient HN03-rep 2 |
| GSM1338422 |
PDAC xenograft from patient A-Nor-rep 1 |
| GSM1338423 |
PDAC xenograft from patient A-Nor-rep 2 |
| GSM1338424 |
PDAC xenograft from patient C-Nor-rep 1 |
| GSM1338425 |
PDAC xenograft from patient C-Nor-rep 2 |
| GSM1338426 |
PDAC xenograft from patient B-Tim-rep 1 |
| GSM1338427 |
PDAC xenograft from patient B-Tim-rep 2 |
| GSM1338428 |
PDAC xenograft from patient D-IPC-rep 1 |
| GSM1338429 |
PDAC xenograft from patient D-IPC-rep 2 |
| GSM1338430 |
PDAC xenograft from patient J-IPC-rep 1 |
| GSM1338431 |
PDAC xenograft from patient J-IPC-rep 2 |
| GSM1338432 |
PDAC xenograft from patient L-IPC-rep 1 |
| GSM1338433 |
PDAC xenograft from patient L-IPC-rep 2 |
| GSM1338434 |
PDAC xenograft from patient AD-IPC-rep 1 |
| GSM1338435 |
PDAC xenograft from patient AD-IPC-rep 2 |
| GSM1338436 |
PDAC xenograft from patient AH-IPC-rep 1 |
| GSM1338437 |
PDAC xenograft from patient AH-IPC-rep 2 |
| GSM1338438 |
PDAC xenograft from patient E-NOR-rep 1 |
| GSM1338439 |
PDAC xenograft from patient E-NOR-rep 2 |
| GSM1338440 |
PDAC xenograft from patient HN-01-rep 1 |
| GSM1338441 |
PDAC xenograft from patient HN-01-rep 2 |
| GSM1338442 |
PDAC xenograft from patient 01-030-rep 1 |
| GSM1338443 |
PDAC xenograft from patient 01-030-rep 2 |
| GSM1338444 |
PDAC xenograft from patient AO-IPC-rep 1 |
| GSM1338445 |
PDAC xenograft from patient AO-IPC-rep 2 |
| GSM1338446 |
PDAC xenograft from patient Foie_8b1-rep 1 |
| GSM1338447 |
PDAC xenograft from patient Foie_8b1-rep 2 |
| GSM1338448 |
PDAC xenograft from patient HN014-rep 1 |
| GSM1338449 |
PDAC xenograft from patient HN014-rep 2 |
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| Relations |
| BioProject |
PRJNA239817 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE55513_RAW.tar |
303.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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