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| Status |
Public on May 22, 2014 |
| Title |
Translational profiling of hypothalamic and midbrain neurons that project to the nucleus accumbens. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Neuroanatomical methods enable high-resolution mapping of neural circuitry, but do not allow systematic molecular profiling of neurons based on their connectivity. Here, we report the development of a novel approach for molecularly profiling projective neurons. We show that ribosomes can be labeled with a camelid nanobody raised against GFP and that this system can be engineered to selectively capture translating mRNAs from cells expressing GFP. We generated a transgenic mouse encoding a nanobody-ribosomal protein fusion (Syn-NBL10) and used a retrograde virus (CAV) encoding GFP to immunoprecipitate ribosomes from projection neurons. This enabled us to profile neurons projecting to the nucleus accumbens. The current method provides a new means for profiling neurons based on their projections.
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| Overall design |
Translating mRNAs immunoprecipitated from neurons projecting to the nucleus accumbens. Each Input and IP sample corrspond to a pooled group of 6 mice.
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| Contributor(s) |
Friedman JM, Ekstrand MI, Nectow AR |
| Citation(s) |
24855954 |
| Submission date |
Mar 11, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Jeffrey M Friedman |
| Organization name |
The Rockefeller University
|
| Department |
Molecular Genetics
|
| Lab |
Friedman Lab
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA241023 |
| SRA |
SRP039970 |
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