|
| Status |
Public on Jul 24, 2014 |
| Title |
Genome-wide mapping of yeast RNA polymerase II termination |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using a modification of the yeast anchor away and PAR-CLIP protocols, we have mapped the position of Pol II genome-wide in living yeast cells after depletion of components of either the polyadenylation (pA) or non-pA termination complexes. Depletion of Ysh1 from the nucleus does not lead to readthrough transcription. In contrast, depletion of the termination factor Nrd1 leads to widespread runaway elongation of non-pA transcripts. Depletion of Sen1 also leads to readthrough at non-pA terminators but in contrast to Nrd1 this readthrough appears less processive.
|
| |
|
| Overall design |
Examination of PAR-CLIP sequencing data of Pol II after removal of different transcriptional termination factors.
|
| |
|
| Contributor(s) |
Schaughency P, Corden J, Merran J |
| Citation(s) |
25299594 |
| Submission date |
Apr 01, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Jeff Corden |
| E-mail(s) |
jcorden@jhmi.edu
|
| Organization name |
JHU
|
| Department |
Molecular Bio and Gen
|
| Lab |
Corden Lab
|
| Street address |
725 N Wolfe Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21211 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
|
| Samples (5)
|
| GSM1361506 |
Nrd1-FRB Without Rapamycin (Rep 1 and 2) |
| GSM1361507 |
Nrd1-FRB With Rapamycin (Rep 1 and 2) |
| GSM1361508 |
Rpb2-HTB Control With Rapamycin (Rep 1 and 2) |
|
| Relations |
| BioProject |
PRJNA243299 |
| SRA |
SRP040778 |
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