|
| Status |
Public on Apr 15, 2014 |
| Title |
Diverse intracellular pathogens activate Type III Interferon expression from peroxisomes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Detection of viral RNA in the cytosol of infected cells depends on RIG-I-lilke recptors that use MAVS as an adapter protein to activate antiviral gene expression. MAVS is
tail-anchored on mitochondria, mitochondria-associated membranes and peroxisomes. As peroxisomal membrane proteins can be mistargeted to mitochondria in the absence
of peroxisomes, we hypothesized that MAVS-dependent antiviral gene expression is activated more efficiently in this instance. Pex19 deficient skin firoblasts derived from a
Zellweger syndrome patient lack peroxisomes. We introduced wild type Pex19 into these cells to restore peroxisome formation and assessed alterations in
the gene expression profile of these cells after reovirus infection.
|
| |
|
| Overall design |
6 samples: 9 and 16 hrs after reovirus infection at an MOI of 100 total RNA was extracted from Pex19 reconstituted and deficient cell lines. Gene expression data was compared to uninfected cells.
|
| |
|
| Contributor(s) |
Dixit E, Kagan JC |
| Citation(s) |
24952503 |
| Submission date |
Apr 14, 2014 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Jonathan Kagan |
| Organization name |
Children's Hospital Boston
|
| Street address |
300 Longwood Ave, Enders 649
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA244580 |
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