|
| Status |
Public on May 01, 2014 |
| Title |
Gene expression profile of DLL4+ and DLL4- Hemato-Endothelial Progenitors (HEPs) subpopulations |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In hESCs, expression of the Notch ligand DLL4 parallels the emergence of bipotent hematoendothelial progenitors (HEPs) and promotes their hematopoietic differentiation. During differentiation, DLL4 is only expressed in a subpopulation of HEPs. To study the developmental fate of the two subpopulations of HEPs identified by DLL4 expression, we FACS-isolated DLL4high and DLL4low/- HEPs at day 15 of differentiation and performed gene expression analysis using microarrays
|
| |
|
| Overall design |
10^5 DLL4high and DLL4low/- HEPs purified by FACS from H9 and AND1 hEBs at day 15 of hematopoietic differentiation were used for gene expression analysis using Whole Human Genome Oligo Microarray chips (Agilent Technologies).
|
| |
|
| Contributor(s) |
Ayllon V, Menendez P |
| Citation(s) |
25778099 |
| Submission date |
Apr 17, 2014 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Veronica Ayllon |
| E-mail(s) |
puzzle_nika@yahoo.es
|
| Organization name |
GENYO. Centre for Genomics and Oncological Research
|
| Street address |
Av Ilustracion, 114
|
| City |
Granada |
| ZIP/Postal code |
18016 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA244867 |
Less...
More...