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| Status |
Public on Aug 02, 2014 |
| Title |
Identification of eQTLs for Hepatic Xbp1s and Socs3 Gene Expression in Mice fed a High-Fat, High Caloric Diet |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Non-alcoholic fatty liver disease (NAFLD) is a highly prevalent form of hepatic disease and feeding mice a High-Fat, High-Caloric (HFHC) diet is a standard model of NAFLD. In order to better understand the genetic basis of NAFLD, we conducted an expression quantitative trait locus (eQTL) analysis of mice fed a HFHC diet. 265 (A/J × C57BL/6J) F2 male mice were fed a HFHC diet for 8 weeks. QTL analysis was utilized to identify genomic regions that regulate hepatic gene expression of Xbp1s and Socs3. We identified two overlapping loci for Xbp1 and Socs3 on Chr 1 (164.0-185.4 Mb and 174.4-190.5 Mb, respectively) and Chr 11 (41.1-73.1 Mb and 44.0-68.6 Mb, respectively), and an additional locus for Socs3 on Chr 12 (109.9-117.4 Mb). C57BL/6J-Chr 11A/J/ NaJ mice fed a HFHC diet manifested the A/J phenotype of increased Xbp1s and Socs3 gene expression (p<0.05), while C57BL/6J-Chr 1A/J/ NaJ mice retained the C57BL/6 phenotype. In addition, we replicated the eQTLs on Chr 1 and 12 (LOD scores ? 3.5) using mice from the BXD murine reference panel challenged with CCl4 to induce chronic liver injury and fibrosis. We have identified overlapping eQTLs for Xbp1 and Socs3 on Chr 1 and 11, and consomic mice confirmed that replacing the C57BL/6 with the A/J Chr 11 resulted in an A/J phenotype for Xbp1 and Socs3 gene expression. Identification of the genes for these eQTLs will lead to a better understanding of the genetic factors responsible for NAFLD and potentially other hepatic diseases.
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| Overall design |
Liver samples from eight-week-old AJ and C57BL6J males fed a High Fat High Calorie diet.
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| Citation(s) |
25617409 |
| Submission date |
Apr 23, 2014 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Richard Green |
| Organization name |
Northwestern University
|
| Street address |
675 N Saint Clair
|
| City |
Chicago |
| ZIP/Postal code |
60611 |
| Country |
USA |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (16)
|
| GSM1372631 |
Eight weeks AJ strain mouse liver sample 1 |
| GSM1372632 |
Eight weeks AJ strain mouse liver sample 2 |
| GSM1372633 |
Eight weeks AJ strain mouse liver sample 3 |
| GSM1372634 |
Eight weeks AJ strain mouse liver sample 4 |
| GSM1372635 |
Eight weeks AJ strain mouse liver sample 5 |
| GSM1372636 |
Eight weeks AJ strain mouse liver sample 6 |
| GSM1372637 |
Eight weeks AJ strain mouse liver sample 7 |
| GSM1372638 |
Eight weeks AJ strain mouse liver sample 8 |
| GSM1372639 |
Eight weeks C57BL6J strain mouse liver sample 1 |
| GSM1372640 |
Eight weeks C57BL6J strain mouse liver sample 2 |
| GSM1372641 |
Eight weeks C57BL6J strain mouse liver sample 3 |
| GSM1372642 |
Eight weeks C57BL6J strain mouse liver sample 4 |
| GSM1372643 |
Eight weeks C57BL6J strain mouse liver sample 5 |
| GSM1372644 |
Eight weeks C57BL6J strain mouse liver sample 6 |
| GSM1372645 |
Eight weeks C57BL6J strain mouse liver sample 7 |
| GSM1372646 |
Eight weeks C57BL6J strain mouse liver sample 8 |
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| Relations |
| BioProject |
PRJNA245390 |
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