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| Status |
Public on Nov 30, 2015 |
| Title |
Gfi1 proteins regulate the emergence of HSCs and blood precursors in the AGM through recruitment of LSD1 [HTS] |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
Gfi1:Dam and Gfi1b:Dam binding analysis of sorted hemogenic endothelial cells (TIE2/C-KIT)
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| Overall design |
The cDNAs encoding DAM-GFI1 and DAM-GFI1B fusion proteins or a DAM control were inserted in a Dox inducible HPRT docking site in ainv18 ES cells (Kyba et al, 2002). Following differentiation, HE cell populations were isolated, genomic DNA prepared and digested with DpnI. The methylated DNA fragments were amplified as previously described {Orian et al, 2009, Vogel et al, 2007) and sequenced on a Illumina HiSeq platform
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| Contributor(s) |
Thambyrajah RS, Mazan M, Patel R, Moignard V, Stefanska M, Clapes T, Möröy T, Robin C, Cowley S, Göttgens B, Kouskoff V, Lacaud G |
| Citation(s) |
26619147 |
| Submission date |
May 02, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Roshana S Thambyrajah |
| E-mail(s) |
Roshana.Thambyrajah@cruk.manchester.ac.uk
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| Organization name |
CRUK Manchester
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| Department |
Stem Cell Biology
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| Street address |
Wilmslow Road
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| City |
Manchester |
| ZIP/Postal code |
M20 4BX |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE57251 |
Gfi1 proteins regulate the emergence of HSCs and blood precursors in the AGM through recruitment of LSD1 |
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| Relations |
| BioProject |
PRJNA246690 |
| SRA |
SRP041873 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE57248_rthambyrajah_Gfi1damID_and_Gfi1bdamID_gene_lists.xlsx.gz |
677.5 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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