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| Status |
Public on Jun 29, 2015 |
| Title |
Novel culture conditions facilitating the induction of naïve pluripotency in human embryonic stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
We report a novel culture condition inducing naive pluripotency in hESCs in a rapid, robust and reproducible way. These naive hESCs were similar to mESCs exhibiting domed colony morphology, increased single survival, reduced doubling time, upregulation of naive pluripotency-specific genes, unbiased lineage-specific differentiation, hypomethylation, separate clustering profile from parental primed hESCs and were dependent on signalling pathways similar to naive mESCs.
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| Overall design |
Global DNA methylation analysis of naive hESCs and their parental primed hESC counterparts.
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| Contributor(s) |
Duggal G, Ghimire S, Brokaert D, Deroo T, Van der Jeught M, Warrier S, Lierman S, Peelman L, Van Soom A, Menten B, Mestdagh P, Vandesompele J, Roost M, Slieker RC, Heijmans BT, Deforce D, De Sutter P, Chuva De Sousa Lopes S, Heindryckx B |
| Citation(s) |
26108678 |
| Submission date |
May 05, 2014 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Nannan He |
| E-mail(s) |
N.He@lumc.nl
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| Organization name |
Leiden University Medical Center
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| Street address |
Einthovenweg 20
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| City |
Leiden |
| ZIP/Postal code |
2333 ZC |
| Country |
Netherlands |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA246240 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE57318_RAW.tar |
183.1 Mb |
(http)(custom) |
TAR |
| GSE57318_methylated_unmethylated_signals_after_quality_control.txt.gz |
15.1 Mb |
(ftp)(http) |
TXT |
| GSE57318_methylated_unmethylated_signals_unfiltered.txt.gz |
16.0 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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