|
| Status |
Public on May 09, 2015 |
| Title |
Evaluation of RNA amplification and RNA-Seq library preparation protocols for spermatozoa RNA profiling |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-Seq technique was applied to investigate the effects of four cDNA amplification kits and two RNA-Seq library preparation kits to the deep sequencing results at different perspectives.
|
| |
|
| Overall design |
The same set of semen samples were applied to investigate the qualitative and quantitative effect of four cDNA amplification methods and two RNA-Seq library preparation methods on sperm transcript profiling.
|
| |
|
| Contributor(s) |
Mao S, Sendler E, Goodrich RJ, Hauser R, Krawetz SA |
| Citation(s) |
25077492 |
| Submission date |
May 09, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Stephen Krawetz |
| E-mail(s) |
steve@compbio.med.wayne.edu
|
| Phone |
313-577-6770
|
| Organization name |
Wayne State University School of Medicine
|
| Department |
Center for Molecular Medicine and Genetics
|
| Lab |
C.S. Mott Center for Human Growth and Development
|
| Street address |
275 E. Hancock
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48201 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA246558 |
| SRA |
SRP041833 |
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