|
| Status |
Public on Jun 29, 2014 |
| Title |
Id2 and Id3 maintain the regulatory T cell pool to suppress inflammatory disease |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Regulatory T (Treg) cells suppress the development of inflammatory disease, but our knowledge of transcriptional regulators that control this function remains incomplete. Here we show that expression of Id2 and Id3 in Treg cells was required to suppress development of fatal inflammatory disease. We found that T cell antigen receptor (TCR)-driven signaling initially decreased the abundance of Id3, which led to the activation of a follicular regulatory T (TFR) cell–specific transcription signature. However, sustained lower abundance of Id2 and Id3 interfered with proper development of TFR cells. Depletion of Id2 and Id3 expression in Treg cells resulted in compromised maintenance and localization of the Treg cell population. Thus, Id2 and Id3 enforce TFR cell checkpoints and control the maintenance and homing of Treg cells.
|
| |
|
| Overall design |
Treg mRNA profiles in lymph node from 3-week-old Id2fl/flId3fl/fl;Foxp3Cre/Cre (Id2 Id3 double-knockout) and control mice are generated by deep sequencing.
|
| |
|
| Contributor(s) |
Miyazaki M, Miyazaki K, Chen S, Itoi M, Miller M, Lu LF, Varki N, Chang AN, Broide DH, Murre C |
| Citation(s) |
24973820 |
| Submission date |
May 14, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Kazuko Miyazaki |
| E-mail(s) |
kamiyazaki@ucsd.edu
|
| Organization name |
University of California, San Diego
|
| Department |
Division of Biological Sciences
|
| Lab |
Cornelis Murre
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0377 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (7)
|
|
| Relations |
| BioProject |
PRJNA247679 |
| SRA |
SRP041997 |
Less...
More...