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| Status |
Public on May 01, 2017 |
| Title |
Genome-wide maps of the total and lysine methylated enriched pools of peroxisome proliferating activated receptor gamma (pparg) co-activator 1 alpha (ppargc1a) in Hepa 1-6 cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report the application of single nucleotide sequencing technology for high-throughput profiling of ppargc1a in mouse hepatome cells (Hepa1-6). By obtaining 1.2 billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide maps of ppargc1a sin mouse hepatoma cells. We identify novel non- coding sites of ppargc1a occupation in hepatic cells for Sirt5, Idh3b, Pfkl, and Hmox2 strongly corresponds with the enrichment of enhancer -associated RNAs.
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| Overall design |
Examination of the genomic disttribution of the total pool of ppargc1a and the lysine methylated (K779me) enriched pool of ppargc1a in mouse Hepa1-6 hepatoma cells.
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| Contributor(s) |
Aguilo F, Zhang F, Zhang W, Walsh M |
| Citation(s) |
26774474 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 CA154809 |
Non-coding RNAs for Epigenetic Transcriptional Silencing in Prostate Cancer |
MOUNT SINAI SCHOOL OF MEDICINE |
Walsh |
| R01 HL103967 |
Chromatin Dynamics of the CFTR locus |
MOUNT SINAI SCHOOL OF MEDICINE |
Walsh |
|
| Submission date |
May 20, 2014 |
| Last update date |
Jul 29, 2019 |
| Contact name |
Martin J. Walsh |
| E-mail(s) |
martin.walsh@mssm.edu
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| Organization name |
Mount Sinai School of Medicine
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| Department |
Structural and Chemical Biology
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| Street address |
One Gustave L. Levy Pl.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA248238 |
| SRA |
SRP042130 |
