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| Status |
Public on Oct 01, 2015 |
| Title |
Serial RNA-seq of a single human |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Psychiatric disorders are characterized by major fluctuations in psychological function over the course of weeks and months, but the dynamic characteristics of human brain function over this timescale in healthy individuals are unknown. Over a period of 18 months, we performed intensive phenome-wide assessment of a single human, including brain connectivity using resting fMRI, measurements of psychological and physical health, and transcriptomic and metabolomic profiling.
Brain connectivity varied across sessions in relation to behavioral variables including mood, fatigue, and food/caffeine intake, as well as variables related to inflammation and gut health. Pathway-based analysis of gene expression in peripheral lymphocytes identified associations with physical health and brain connectivity, including associations between specific brain networks and a broad set of immune-related pathways. Metabolomic measures were strongly associated with dietary variance.
This study integrates dense neuroimaging and -omics profiling to provide a detailed picture of the joint dynamics of human brain and metabolic function over time, an approach that is critical for the understanding of brain disorders characterized by increased variability of brain function.
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| |
|
| Overall design |
Repeated measures on a single individual;
RNA was extracted from PBMCs from a single human repeatedly over the course of 18 months, under fasted conditions at a consistent time of day. This was collected along side a large number of other variables including brain imaging.
More information is available at http://www.myconnectome.org
|
| Web link |
http://myconnectome.org/
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| |
|
| Contributor(s) |
Poldrack R |
| Citation(s) |
26648521 |
| Submission date |
May 30, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Russell Poldrack |
| E-mail(s) |
poldrack@gmail.com
|
| Phone |
15122329504
|
| Organization name |
University of Texas at Austin
|
| Street address |
100 East 24th Street, R9975
|
| City |
Austin |
| State/province |
TX |
| ZIP/Postal code |
78712 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (48)
|
|
| Relations |
| BioProject |
PRJNA249059 |
| SRA |
SRP042596 |
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