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| Status |
Public on Feb 04, 2015 |
| Title |
Genome-wide Oct4 binding profile in lung cancer A549 cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Oct4 stemness gene encoding a transcription factor has been shown to overexpress in cancers. However, precise mechanisms of Oct4 relevant to transcriptional reprogramming leading to somatic cancer progression remain unclear. To address the Oct4-mediated transcriptional program in lung cancer, we integrated genome-wide Oct4 binding profiles from chromatin-immunoprecipitation sequencing and ENCODE datasets. We identified that Oct4 occupied at functional promoter and enhancer regions of genes which play key roles in several signaling pathways involving tumorigenesis.
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| |
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| Overall design |
Genome-wide Oct4 binding sites were identified via chromatin immunoprecipitation-sequencing analysis of vecoter control and stably Oct4-overexpressing A549 lung cancer cells. ChIP-seq analyses were performed in duplicated samples using Applied Biosystems SOLiD system.
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| |
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| Contributor(s) |
Tang Y, Sun HS, Wang Y |
| Citation(s) |
25609695 |
| Submission date |
Jun 13, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Yi-Ching Wang |
| E-mail(s) |
ycw5798@mail.ncku.edu.tw
|
| Phone |
+886-6-2353535
|
| Organization name |
National Cheng Kung University
|
| Department |
Department of Pharmacology, College of Medicine
|
| Street address |
No.1, University Road, Tainan 70101, Taiwan, R. O. C.
|
| City |
Tainan |
| ZIP/Postal code |
70101 |
| Country |
Taiwan |
| |
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| Platforms (1) |
| GPL16288 |
AB 5500xl Genetic Analyzer (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA253167 |
| SRA |
SRP043451 |
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