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| Status |
Public on Dec 10, 2014 |
| Title |
A Novel Human Gastric Primary Cell Culture System for Modeling Helicobacter Pylori Infection In Vitro |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We identified specific long-term culture conditions that allow the formation of 3-dimensional human gastric spheroids, which can be differentiated by withdrawal of Wnt3A and R-spondin1 to human gastric organoids. These cultures can expand indefinitely and exhibit important characteristics of human stomach tissue. Furthermore, these 3-dimensional cultures can be transferred into 2-dimensional primary epithelial cell layers, which can be successfully infected with H. pylori and thereby provide ideal conditions to study infections in vitro.
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| Overall design |
Microarray experiments were performed as dual-color hybridizations on Agilent human whole genome catalog 44K arrays. To compensate for dye-specific effects, a dye-reversal color-swap was applied.
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| Contributor(s) |
Schlaermann P, Meyer T, Mollenkopf H |
| Citation(s) |
25539675 |
| Submission date |
Jun 13, 2014 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Hans-Joachim Mollenkopf |
| E-mail(s) |
mollenkopf@mpiib-berlin.mpg.de
|
| Phone |
+49 30 28460 482
|
| Organization name |
Max-Planck-Institute for Infection Biology
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| Lab |
Microarray/Genomics Core Facility
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| Street address |
Charitéplatz 1
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| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
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| Platforms (2) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
| GPL10332 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Feature Number version) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA252707 |
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