|
| Status |
Public on Dec 04, 2014 |
| Title |
Uridylation by TUT4 and TUT7 marks mRNA for degradation [RNA-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Uridylation occurs pervasively on mRNAs in mammals, yet its mechanism and significance remain unknown. Here we identify TUT4 and TUT7 (also known as ZCCHC11 and ZCCHC6, respectively) as the enzymes that uridylate mRNAs. Uridylation readily occurs on deadenylated mRNAs that are not associated with poly(A) binding protein (PABPC1) in cells. Consistently, purified TUT4 and TUT7 (TUT4/7) selectively uridylate RNAs with short A tails (< ~25 nt) while PABPC1 antagonizes uridylation of polyadenylated mRNAs in vitro. In cells depleted of TUT4/7, the vast majority of mRNAs lose the U tails, and their half-lives are extended. Suppression of mRNA decay factors leads to the accumulation of uridylated mRNAs. In line with this, microRNA induces uridylation of its targets, and TUT4/7 is required for enhanced decay of microRNA targets. Our study explains the mechanism underlying selective uridylation of deadenylated mRNAs, and demonstrates a fundamental role of the U tail as a molecular mark for global mRNA decay.
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| Overall design |
HeLa cells were knocked down of control or TUT4/7, then total RNAs were prepared for RNA-seq on 0, 1, 2, 4h after actinomycin D treatment. The whole processes of experiments were repeated two times.
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| Contributor(s) |
Lim J, Ha M, Chang H, Kim VN |
| Citation(s) |
25480299 |
| Submission date |
Jul 21, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Hyeshik Chang |
| E-mail(s) |
hyeshik@snu.ac.kr
|
| Organization name |
Seoul National University
|
| Department |
School of Biological Sciences
|
| Lab |
Hyeshik Chang Lab
|
| Street address |
Building 203 Room 525, School of Biological Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu
|
| City |
Seoul |
| State/province |
South Korea |
| ZIP/Postal code |
08826 |
| Country |
South Korea |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (16)
|
| GSM1440658 |
RNA-seq siCont ActD 4h |
| GSM1440659 |
RNA-seq siTUT47 ActD 0h |
| GSM1440660 |
RNA-seq siTUT47 ActD 1h |
| GSM1440661 |
RNA-seq siTUT47 ActD 2h |
| GSM1440662 |
RNA-seq siTUT47 ActD 4h |
| GSM1514008 |
RNA-seq siCont ActD 0h (biological replicate #2) |
| GSM1514009 |
RNA-seq siCont ActD 1h (biological replicate #2) |
| GSM1514010 |
RNA-seq siCont ActD 2h (biological replicate #2) |
| GSM1514011 |
RNA-seq siCont ActD 4h (biological replicate #2) |
| GSM1514012 |
RNA-seq siTUT47 ActD 0h (biological replicate #2) |
| GSM1514013 |
RNA-seq siTUT47 ActD 1h (biological replicate #2) |
| GSM1514014 |
RNA-seq siTUT47 ActD 2h (biological replicate #2) |
| GSM1514015 |
RNA-seq siTUT47 ActD 4h (biological replicate #2) |
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| This SubSeries is part of SuperSeries: |
| GSE59628 |
Uridylation by TUT4 and TUT7 marks mRNA for degradation |
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| Relations |
| BioProject |
PRJNA255776 |
| SRA |
SRP044679 |
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