|
| Status |
Public on Oct 01, 2014 |
| Title |
Nova1 is a master regulator of alternative splicing in pancreatic beta cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Alternative splicing (AS) is a fundamental mechanism for the regulation of gene expression. It affects more than 90% of human genes but its role in the regulation of pancreatic beta cells, the producers of insulin, remains unknown. Our recently published data indicated that the “neuron specific” Nova1 splicing factor is expressed in pancreatic beta cells. We have presently coupled specific knockdown (KD) of Nova1 with RNA-sequencing to determine all splice variants and downstream pathways regulated by this protein in beta cells. Nova1 KD altered the splicing of nearly 5000 transcripts. Pathway analysis indicated that these genes are involved in exocytosis, apoptosis, insulin receptor signalling, splicing and transcription. In line with these findings, Nova1 silencing inhibited insulin secretion and induced apoptosis basally and after cytokine treatment in rodent and human beta cells. These observations identify a novel layer of regulation of beta cell function, namely AS controlled by key splicing regulators such as Nova1.
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| |
|
| Overall design |
3 batch of primary rat pancreatic beta cells were examined under 2 conditions: control and with Nova1 splicing factor knock-down
|
| |
|
| Contributor(s) |
Villate O, Turatsinze J, Sammeth M, Eizirik DL |
| Citation(s) |
25249621 |
| Submission date |
Jul 21, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Olatz Villate |
| E-mail(s) |
Olatz.Villate@ulb.ac.be
|
| Organization name |
Universite Libre de Bruxelles
|
| Department |
Faculty of Medecine
|
| Lab |
Laboratory of Experimental Medecine and ULB Center of Diabetes Research
|
| Street address |
Route de Lennik 808 CP618
|
| City |
Bruxelles |
| ZIP/Postal code |
1070 |
| Country |
Belgium |
| |
|
| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
|
| Samples (6)
|
| GSM1440963 |
rat pancreatic primary beta cell batch 1 under control condition |
| GSM1440964 |
rat pancreatic primary beta cell batch 1 with Nova1 knock-down |
| GSM1440965 |
rat pancreatic primary beta cell batch 2 under control condition |
| GSM1440966 |
rat pancreatic primary beta cell batch 2 with Nova1 knock-down |
| GSM1440967 |
rat pancreatic primary beta cell batch 3 under control condition |
| GSM1440968 |
rat pancreatic primary beta cell batch 3 with Nova1 knock-down |
|
| Relations |
| BioProject |
PRJNA255786 |
| SRA |
SRP044684 |
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