|
| Status |
Public on Jul 20, 2015 |
| Title |
N6-adenosine methylation in miRNAs |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
Other
|
| Summary |
Methylation of N6-adenosine (me6A) has been observed in rRNA, tRNA, snoRNA, lncRNA and mRNA (ref.), but not yet miRNA. Interestingly, many mRNAs contain miRNA-binding sites and me6A in their 3'-UTR, although they do not appear to overlap (Mayer). We have investigated whether adenosine methylation affects miRNA levels and whether miRNAs are methylated. Indeed, we have obtained evidence suggesting that miRNA can be methylated and that the steady state level of certain miRNAs is affected by the level of the 6meA demethylase FTO.
|
| |
|
| Overall design |
RNA-Seq of small RNAs prepared from FTO knockdown cells. RNA-Seq of small RNA fraction first immunoprecipitated by antibody against me6A
|
| |
|
| Contributor(s) |
Berulava T, Rademacher K, Klein-Hitpass L, Rahmann S, Horsthemke B |
| Citation(s) |
25723394 |
| Submission date |
Jul 25, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Ludger Klein-Hitpass |
| E-mail(s) |
ludger.klein-hitpass@uni-essen.de
|
| Phone |
+49 201 723 85552
|
| Organization name |
Institut fuer Zellbiologie
|
| Department |
Universitaetsklinikum
|
| Lab |
BioChip Lab
|
| Street address |
Virchowstr. 173
|
| City |
Essen |
| ZIP/Postal code |
D-45122 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA256175 |
| SRA |
SRP044847 |
Less...
More...