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| Status |
Public on Jul 29, 2014 |
| Title |
Gene expression profiling associated with knockdown of LKB1 in human intrahepatic cholangiocarcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The experiment was designed to display differential gene expression profiling in three human intrahepatic cholangiocarcinoma (ICC) cells upon knockdow of LKB1 tumor suppressor, by using RNAseq technology.
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| |
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| Overall design |
LKB1 was first attenuated in three ICC cells: HuH-28, RBE, and SSP-25 by siRNA-mediated knockdown. Total RNA was extracted from duplicated cancers cells transfected with control siRNA and LKB1 specific siRNA at 48h posttransfection for RNAseq analysis. Differentially expressed genes in LKB1-attenuated ICC cells were identified in comparison to that in ICC cells transfected with control siRNA.
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| |
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| Contributor(s) |
Zhang K, Yen Y, Wang J, Lu Y, Wu X |
| Citation(s) |
26056085 |
| Submission date |
Jul 28, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
keqiang zhang |
| Organization name |
City of Hope National Medical Center
|
| Department |
Molecular Pharmacology
|
| Street address |
1500 East Duarte Road
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA256469 |
| SRA |
SRP045048 |
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