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| Status |
Public on Jun 26, 2015 |
| Title |
The H3K4-methyl epigenome regulates MLL leukemia stem cell oncogenic potential |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The genetic programs that maintain leukemia stem cell (LSC) self-renewal and oncogenic potential have been well defined, however the epigenetic landscape that determines their cellular identity and functionality has not been established. We report that LSCs in MLL-associated leukemia are maintained in an epigenetic state defined by relative genome-wide high-level H3K4me3 methylation and low level H3K79me2. LSC differentiation is associated with dynamic reversal of these broad epigenetic profiles and concomitant down-regulation of the LSC maintenance transcriptional program. LSCs also share with embryonic stem cells a large subset of genes with bivalent histone marks related to embryonic development. The histone demethylase KDM5B negatively regulates MLL-induced leukemogenesis demonstrating the crucial role of the H3K4 global methylome for determining leukemia stem cell fate.
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| |
|
| Overall design |
Investigation of multiple histone modification marks and RNA Pol II in ckit+ and ckit- cells isolated and fractionated from MLL leukemia mice.
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| |
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| Contributor(s) |
WONG SH, Cleary ML |
| Citation(s) |
26190263 |
| Submission date |
Aug 07, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Stephen HK WONG |
| E-mail(s) |
honkit@stanford.edu
|
| Phone |
6507235340
|
| Organization name |
Stanford University
|
| Department |
Pathology
|
| Lab |
Dr. Michael Cleary
|
| Street address |
G2035 SIM1 Bldg
|
| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA257737 |
| SRA |
SRP045332 |
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