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| Status |
Public on May 20, 2015 |
| Title |
Synthetic mRNA switches for detection and purification of cardiomyocytes and endothelial cells derived from human pluripotent stem cells [mRNA] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We designed microRNA-responsive, modified mRNA switches (miR-switches) that quantitatively control their own translation level by determining the miRNA activities. We found that the three miR-switches (i.e., miR-1-, miR-208-, and miR-499-switches) enable to purify cardiomyocytes differentiated from hPSCs with high efficiency, accuracy, and safety. The purified cardiomyocytes were engrafted in mouse heart and did not form tumor. Simultaneous purification of two different cell types, cardiomyocytes and endothelial cells, was also performed by transfecting the mRNA responding to the both miR-208 and miR-126, into heterogeneous cell population derived from hPSCs. In addition, selective induction of apoptosis in noncardiomyocytes triggered by miR-1/208-Bim switch enriched cardiomyocytes without cell sorting. The mRNA switch could purify desired cell types and control cell death pathways by monitoring the miRNA expression dynamics during cell fate conversion.
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| Overall design |
MYH6-EIP4 iPSCs for mRNA, N=3
MYH6-EIP4 day18 cardiomyocytes (before transfection) for mRNA, N=3
MYH6-EIP4 day19 cardiomyocytes (miR-1-switch day1) for mRNA, N=3
MYH6-EIP4 day19 cardiomyocytes (without transfection) for mRNA, N=3
MYH6-EIP4 day25 cardiomyocytes (miR-1-switch day7) for mRNA, N=3
MYH6-EIP4 day25 cardiomyocytes (without transfection) for mRNA, N=3
201B7 d22 miR-208a-BFP sorted cells for mRNA, N=1
201B7 d22 miR-208a-BFP negative fraction sorted cells for mRNA, N=1
201B7 d22 SIRPA+LIN- sorted cells for mRNA, N=1
201B7 d22 SIRPA+LIN- negative fraction sorted cells for mRNA, N=1
201B7 d22 VCAM1+ sorted cells for mRNA, N=1
201B7 d22 VCAM1+negative fraction sorted cells for mRNA, N=1
MYH6-EIP4 day19 cardiomyocytes (miR-208a-Bim-switch day1) for mRNA, N=1
MYH6-EIP4 day19 cardiomyocytes (without transfection) for mRNA, N=1
MYH6-EIP4 day21 cardiomyocytes (miR-208a-Bim-switch day7) for mRNA, N=1
MYH6-EIP4 day21 cardiomyocytes (without transfection) for mRNA, N=1
MYH6-EIP4 day25 cardiomyocytes (miR-208a-Bim-switch day7) for mRNA, N=1
MYH6-EIP4 day25 cardiomyocytes (without transfection) for mRNA, N=1
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| Contributor(s) |
Miki K, Narita M, Saito H, Yoshida Y |
| Citation(s) |
26004781 |
| Submission date |
Aug 22, 2014 |
| Last update date |
Oct 11, 2016 |
| Contact name |
Yoshinori Yoshida |
| E-mail(s) |
yoshinor@cira.kyoto-u.ac.jp
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| Organization name |
Kyoto University
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| Street address |
53, Kawahara-cho, Shogoin, Sakyo-ku
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| City |
Kyoto |
| ZIP/Postal code |
6068507 |
| Country |
Japan |
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| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (31)
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| This SubSeries is part of SuperSeries: |
| GSE60633 |
Efficient detection and purification of cell populations using synthetic microRNA switches |
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| Relations |
| BioProject |
PRJNA259165 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE60632_RAW.tar |
94.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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